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WTSIi085-A

HPSI1113i-eofe_1

iPSC line

No longer available
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
The cell line was withdrawn.

General#

Cell Line

hPSCreg name WTSIi085-A
Alternative name(s)
HPSI1113i-eofe_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi085-A
BioSamples SAMEA2474456
HipSci HPSI1113i-eofe_1
Cellosaurus CVCL_AH90
Wikidata Q54891623

General Information

Publications View all related publications on hPSCreg (2)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 45-49
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2397886
HipSci HPSI-eofe

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 45-49
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Gelatin
Feeder cells Mouse embryo fibroblast (MEF) feeder cells
Passage method Enzymatically
Collagenase and Dispase
CO2 Concentration 5 %
Medium Other medium:
Base medium:
Main protein source: Knock-out serum replacement

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
33.752 1.439 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1113i-eofe_1
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122978
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947023
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860373
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861237
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859989
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448056
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122912
mpileup variant calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123045
Splice-aware STAR alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267019
Abundances of transcripts
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR946967
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860853
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860757
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127640
Genotyping array calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127405
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR862690
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947019
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861045
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861141
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859893
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860565
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860661
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860469
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860949
Raw sequencing reads
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
breast-ovarian cancer, familial, susceptibility to, 2 (BRCA2)
Charcot-Marie-Tooth disease (FIG4, HINT1)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, LAMC3, SETBP1)
congenital fibrosis of extraocular muscles (KIF21A)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (NPPA)
Fanconi anemia complementation group A (FANCA)
Fanconi anemia complementation group D1 (BRCA2)
focal segmental glomerulosclerosis 9 (CRB2)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 5 (HYDIN)
primary ciliary dyskinesia 7 (DNAH11)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
Usher syndrome type 1 (CDH23)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
Other WGS-derived genes
AGAP6, AGL, ALDH3A2, ATXN3, AURKC, BMP4, CASP12, CHST15, CIZ1, CYP2D6, CYP2F1, CYP3A5, D2HGDH, DEFB126, DSC3, ERCC6L2, FBXO7, FLG2, GLYCTK, HLA-DRB5, IDO2, IRF5, KCNJ16, KISS1, LAMA5, MROH8, NDUFB9, NPRL3, NRP2, OAS1, OR1B1, P2RX5, PDE4DIP, PIGN, POLDIP2, POLR3B, PTCHD3, PTPRN2, RP1L1, RXFP2, SCAPER, SIGLEC12, SLC37A4, SPATA7, SRA1, STAG2, SYNE2, TIGD6, TK2, TMEM107, TMEM216, TMPRSS6, TNRC18, TOR1AIP1, TREH, TRPM1, UGT2A1, VDR, WDR37, ZAN, ZNF142, ZNF440, ZNF527, ZNF83