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WTSIi083-A

HPSI1213i-xuja_2

iPSC line

Not-for-profit fee: £1400 per vial
Stock not immediately available - enquire for details
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

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General#

Cell Line

hPSCreg name WTSIi083-A
Alternative name(s)
HPSI1213i-xuja_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi083-A
BioSamples SAMEA2494988
HipSci HPSI1213i-xuja_2
Cellosaurus CVCL_AI23
Wikidata Q54891734

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 45-49
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2397810
HipSci HPSI-xuja

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 45-49
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
34.569 1.348 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1213i-xuja_2
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914302
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914338
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1901045
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127593
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122925
mpileup variant calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127358
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947057
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123058
Splice-aware STAR alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947065
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447977
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267002
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122991
Imputed and phased genotypes
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
breast-ovarian cancer, familial, susceptibility to, 2 (BRCA2)
Charcot-Marie-Tooth disease (FIG4, HINT1)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
factor XIII, b subunit, deficiency of (F13B)
Fanconi anemia complementation group A (FANCA)
Fanconi anemia complementation group D1 (BRCA2)
Hermansky-Pudlak syndrome 4 (HPS4)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23)
Noonan syndrome (MRAS)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 5 (HYDIN)
primary ciliary dyskinesia 7 (DNAH11)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C, MED13L)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
X-linked complex neurodevelopmental disorder (ZNF711)
Other WGS-derived genes
A2M, ABCA10, AGAP6, AGL, ANAPC1, AURKC, BMP4, BTN3A2, CASP12, CATSPER2, CHIT1, CHST15, CLDN16, CLEC7A, COLQ, CST3, CYP21A2, CYP2D6, CYP4B1, D2HGDH, DEFB126, DNAAF1, DSC3, ERCC6L2, FANCM, FBXO7, FLG2, FUT2, GALNT3, GDPD4, GLYCTK, HSD17B13, ITGB2, KCNJ16, LPA, MESP1, MROH8, MTTP, NDUFB9, OAS1, OPRM1, OR52B4, P2RX5, PDE4DIP, PGM1, PHKB, PIGN, POLDIP2, PTCHD3, PTPRN2, RNF212, RP1L1, RXFP2, SCAPER, SIGLEC12, SLC37A4, SRA1, TBP, TDG, TGIF1, TIGD6, TMEM107, TMEM216, TMPRSS6, TMX3, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZNF83, ZNF844