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WTSIi082-A

HPSI1013i-cups_3

iPSC line

Purchases have been temporarily disabled, as we are transitioning sales and distribution.

If you are interested in purchasing this cell line, please contact EBiSC directly. For more information about the current transition process see here.
The cell line was withdrawn.

General#

Cell Line

hPSCreg Name WTSIi082-A
Alternative name(s)
HPSI1013i-cups_3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi082-A
BioSamples SAMEA2498700
HipSci HPSI1013i-cups_3
Cellosaurus CVCL_AH60
CLO CLO_0101061
Wikidata Q54891541

General Information

Publications View all related publications on hPSCreg (2)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 45-49
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2398406
HipSci HPSI-cups

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 45-49
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Gelatin
Feeder cells Mouse embryo fibroblast (MEF) feeder cells
Passage method Enzymatically
Collagenase and Dispase
CO2 Concentration 5 %
Medium Other medium:
Base medium:
Main protein source: Knock-out serum replacement

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
34.822 1.295 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1013i-cups_3
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267055
Abundances of transcripts
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127327
Imputed and phased genotypes
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127562
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR780159
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR946971
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861177
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861273
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR847013
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR946996
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860793
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859929
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860697
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122932
mpileup variant calls
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861081
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123065
Splice-aware STAR alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122998
Imputed and phased genotypes
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860025
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860505
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860601
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860985
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860409
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860889
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448179
GATK haplotype calls
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (NPPA)
familial ovarian cancer (GEN1)
Fanconi anemia complementation group A (FANCA)
hereditary breast carcinoma (GEN1)
Hermansky-Pudlak syndrome 4 (HPS4)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
mucopolysaccharidosis type 1 (IDUA)
mucopolysaccharidosis type 4A (GALNS)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A, TMPRSS3)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 7 (DNAH11)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
X-linked complex neurodevelopmental disorder (ZNF711)
Other WGS-derived genes
AAGAB, AGAP1, AGL, ALDH3A2, AMPD1, AURKC, BFSP1, BMP4, CARD8, CASP12, CHST15, COLQ, CYP2D6, CYP2F1, CYP4B1, DEFB126, DNAAF1, DSC3, FASTKD1, FBXO7, FLG, FLG2, FUT2, GALNT3, GDPD4, GPRIN1, HSD17B13, IDO2, IL12RB1, IRF5, ITGB2, KCNJ16, KLHL3, KRT18, LAMA5, MAPT, MESP1, MICA, MPO, MROH8, NDUFB9, NOTCH2, NPRL3, NRP2, OR1B1, OR51F1, P2RX5, PDE4DIP, PIGN, POLDIP2, SIGLEC12, SLC37A4, SPATA7, SRA1, SYNE2, TBP, TGIF1, TIGD6, TK2, TMEM107, TMEM175, TMEM216, TMPRSS6, TNRC18, TOR1AIP1, TREH, TRPM1, UTRN, VDR, WDR37, ZAN, ZNF141, ZNF527