WTSIi073-A

HPSI0314i-sojd_3

iPSC line

The cell line was withdrawn.

General#

Cell Line

hPSCreg Name WTSIi073-A
Alternative name(s)
HPSI0314i-sojd_3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi073-A
BioSamples SAMEA2627569
HipSci HPSI0314i-sojd_3
Cellosaurus CVCL_AE87
ECACC 77650126
CLO CLO_0101048
Wikidata Q54890627

General Information

Publications View all related publications on hPSCreg (2)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 45-49
Ethnicity White - Other

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2418245
HipSci HPSI-sojd

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 45-49
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
24.625 1.479 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0314i-sojd_3
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266960
Splice-aware STAR alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1203453
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127401
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203381
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203413
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448150
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203405
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1882668
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266817
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266913
BWA alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267048
Abundances of transcripts
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127636
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1243456
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266723
mpileup variant calls
WGS-derived disease associations
Charcot-Marie-Tooth disease (FIG4, HINT1)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
factor XIII, b subunit, deficiency of (F13B)
Lynch syndrome (PMS1)
mitochondrial disease (NDUFS6)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 2 (DNAAF3)
primary ciliary dyskinesia 7 (DNAH11)
PTEN hamartoma tumor syndrome (PTEN)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
Other WGS-derived genes
A2M, AGAP1, AGAP6, AGL, AHNAK, AKR1E2, ALDH3A2, ANAPC1, AURKC, CASP12, CATSPER2, COLQ, CSH2, CYP21A2, D2HGDH, DEFB126, DNAAF1, DSC3, ERCC6L2, FLG2, FUT2, FZD6, GDPD4, GLYCTK, H6PD, IL12RB1, IRF5, ITGB2, KCNJ16, KISS1, KRT18, LAMA5, LDHB, MAPT, MICA, MROH8, NAGLU, NDUFB9, NFU1, NPRL3, NRP2, OAS1, OR1B1, OR51F1, OR52B4, P2RX5, PDE4DIP, PIGN, POLDIP2, POLR3B, PYGL, RXFP2, SELPLG, SIGLEC12, SLC37A4, SRA1, SYNE2, TAP2, TAS2R7, TGIF1, TLR5, TMEM107, TMEM175, TMEM216, TMPRSS6, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZNF141, ZNF527, ZNF83, ZNF844