WTSIi071-B

HPSI0913i-lise_3

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi071-B
Alternative name(s)
HPSI0913i-lise_3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi071-B
BioSamples SAMEA2625589
HipSci HPSI0913i-lise_3
Cellosaurus CVCL_AH44
ECACC 77650086, 66540193
CLO CLO_0101046
Wikidata Q54891470

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 45-49
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398439
HipSci HPSI-lise

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 45-49
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Passage method EDTA
Surface coating Vitronectin
O2 concentration 20
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Pluripotency Score Novelty Score Link to microarray data
28.62 1.333 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0913i-lise_3
Number of regions different from primary tissue: 21; Length of differences from primary tissue: 20
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127373
Imputed and phased genotypes
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448023
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267012
Abundances of transcripts
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127608
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122921
mpileup variant calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914301
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947055
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914337
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861345
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123054
Splice-aware STAR alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947063
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122987
Imputed and phased genotypes
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
WGS-derived disease associations
Charcot-Marie-Tooth disease (FIG4)
Clouston syndrome (GJB6)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (NPPA)
familial ovarian cancer (GEN1)
Fanconi anemia complementation group A (FANCA)
hereditary breast carcinoma (GEN1)
hereditary nonpolyposis colon cancer (XRCC4)
Lynch syndrome (PMS1)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (GJB6, MYO7A)
Noonan syndrome (A2ML1)
primary ciliary dyskinesia (DNAH8)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
spermatogenic failure 46 (DNAH8)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
X-linked complex neurodevelopmental disorder (SHROOM4)
Other WGS-derived genes
ACTR3C, AGAP1, AGAP6, AGL, ATXN3, AURKC, BMP4, CARD8, CASP12, CATSPER2, CHIT1, CHST15, COLQ, CYP21A2, D2HGDH, DEFB126, DSC3, DUSP6, FCGR2A, FMO2, GALNT3, GLYCTK, GPR161, H6PD, HSD17B13, IL12RB1, IRF5, ITGB2, KCNJ16, LAMA5, MESP1, MROH8, NDUFB9, NFU1, NOTCH2, NPRL3, OAS1, OPRM1, OR1B1, OR51F1, P2RX5, PDE4DIP, PIGN, PITX2, POLDIP2, POLR3B, PRMT7, PTCHD3, PYGL, RP1L1, SLC37A4, SPATA7, SYNE2, TAP2, TIGD6, TMEM107, TMEM216, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZAN, ZNF233