Please take our EBiSC Customer Survey.

WTSIi052-A

HPSI1114i-ziyn_6

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi052-A
Alternative name(s)
HPSI1114i-ziyn_6
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi052-A
BioSamples SAMEA3311866
HipSci HPSI1114i-ziyn_6
Cellosaurus CVCL_AI11
ECACC 77650206, 66540046
CLO CLO_0101018
Wikidata Q54891684

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 60-64
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA3109703
HipSci HPSI-ziyn

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 60-64
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
28.932 1.33

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1114i-ziyn_6
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 0
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266945
Splice-aware STAR alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1243474
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858764
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858860
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858668
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266989
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203399
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858380
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858188
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858284
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266866
BWA alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266676
mpileup variant calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1203471
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368848
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858956
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447918
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203431
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369140
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858572
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858476
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266770
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203447
Raw sequencing reads
WGS-derived disease associations
Charcot-Marie-Tooth disease (FIG4, HINT1)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (SETBP1)
congenital myopathy (MYL1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
factor XIII, b subunit, deficiency of (F13B)
familial ovarian cancer (GEN1)
hereditary breast carcinoma (GEN1)
Huntington disease (HTT)
Lynch syndrome (PMS1)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
primary ciliary dyskinesia 7 (DNAH11)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
Other WGS-derived genes
ACTR3C, AGAP1, AGL, AKR1E2, ALDH3A2, ARMS2, AURKC, BTNL2, CARD8, CASP12, CATSPER2, COLQ, CYP2D6, CYP4B1, DEFB126, DNAAF1, DNAH9, DNAJA4, DSC3, ERCC6L2, FBXO7, FCGR2A, FUT2, FZD6, GALNT3, GLYCTK, H6PD, HLA-DRB5, IRF5, ITGB2, KCNJ16, KISS1, KLHL3, LAMA5, MAPT, MROH8, NDUFB9, OAS1, OR1B1, P2RX5, PDE4DIP, PIGN, POLDIP2, POLR3B, PRKRA, PTCHD3, RNF212, SLC37A4, SRA1, SYNE2, TBP, TGIF1, THEMIS, TIGD6, TMEM107, TMEM216, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZAN, ZNF124, ZNF528, ZNF99