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WTSIi049-A

HPSI0514i-wiii_2

iPSC line

Not-for-profit fee: £1400 per vial
Stock not immediately available - enquire for details
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

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General#

Cell Line

hPSCreg name WTSIi049-A
Alternative name(s)
HPSI0514i-wiii_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi049-B
(HPSI0514i-wiii_3)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi049-A
BioSamples SAMEA3312840
HipSci HPSI0514i-wiii_2
Cellosaurus CVCL_AF85
Wikidata Q54890934

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 65-69
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2554407
HipSci HPSI-wiii

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 65-69
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
17.866 1.351

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0514i-wiii_2
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266880
BWA alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266690
mpileup variant calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266784
Imputed and phased genotypes
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369068
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858656
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858752
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1274910
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1279070
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858176
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858272
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1279106
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858848
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858944
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266950
Splice-aware STAR alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1279092
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368776
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858464
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858368
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858560
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447966
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267000
Abundances of transcripts
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1274918
Raw sequencing reads
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Charcot-Marie-Tooth disease (FIG4)
classic homocystinuria (CBS)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (LAMC3, SETBP1)
hyperprolinemia type 1 (PRODH)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (ABCC8, PAX4)
mucopolysaccharidosis type 4A (GALNS)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
Parkinson disease (LRRK2)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 7 (DNAH11)
PTEN hamartoma tumor syndrome (PTEN)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
X-linked complex neurodevelopmental disorder (ZNF711)
Other WGS-derived genes
AGL, AGXT2, ALDH3A2, ARMC5, AURKC, CASP12, CATSPER2, CEP170, CHIT1, CLDN16, CNOT1, COLQ, CYP21A2, CYP2D6, CYP3A5, CYP4B1, DEFB126, DNAAF1, DSC3, ERCC6L2, FBXO7, FCGR2A, FUT2, GALNT3, GDPD4, GLYCTK, GYS1, HLA-DRB5, IDO2, IFIH1, ITGB2, KCNJ16, KISS1, LAMA5, MESP1, MICA, MROH8, MTTP, NCOA3, NDUFB9, NPRL3, OAS1, OPRM1, OR1B1, OR51F1, OTOR, P2RX5, PDE4DIP, PIGN, PITX2, POLDIP2, PRKRA, PYGL, RXFP2, SIGLEC12, SLC37A4, SRA1, TBP, TIGD6, TMEM107, TMEM216, TMPRSS6, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZAN, ZNF141, ZNF233