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WTSIi047-B

HPSI0814i-bokz_6

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi047-B
Alternative name(s)
HPSI0814i-bokz_6
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi047-B
BioSamples SAMEA3355550
HipSci HPSI0814i-bokz_6
Cellosaurus CVCL_AH23
ECACC 77650210, 66540122
CLO CLO_0101012
Wikidata Q54891421

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 55-59
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2724752
HipSci HPSI-bokz

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 55-59
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
18.05 0.917

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0814i-bokz_6
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203402
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858441
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858345
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858249
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267068
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266936
BWA alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266746
mpileup variant calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266969
Splice-aware STAR alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1243477
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203450
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369194
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858537
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858633
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368902
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858153
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266840
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203434
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1203474
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858921
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858729
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1858825
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448228
GATK haplotype calls
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
arrhythmogenic right ventricular cardiomyopathy (TTN)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (NPPA, TTN)
familial isolated arrhythmogenic right ventricular dysplasia (DSC2)
familial ovarian cancer (GEN1)
Fanconi anemia complementation group A (FANCA)
hereditary breast carcinoma (GEN1)
hypertrophic cardiomyopathy (TTN)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
myopathy, myofibrillar, 9, with early respiratory failure (TTN)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
PHARC syndrome (ABHD12)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
tibial muscular dystrophy (TTN)
TTN-related myopathy (TTN)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
Other WGS-derived genes
ABCA10, ACD, AGAP1, AGAP6, AGL, ALDH3A2, ANAPC1, AURKC, BTNL2, CARD8, CASP12, CASP8, CEP170, CHIT1, CHST15, COLQ, CYP4B1, DEFB126, DNAAF1, DSC3, ERCC6L2, FBXO7, FLG2, FUT2, GAL3ST2, GALNT3, GDPD4, GIGYF2, GLYCTK, H6PD, HSD17B13, IDO2, INMT, IRF5, ITGB2, KCNJ16, KRT83, LAMA5, MAPT, MICA, MROH8, NDUFB9, NFU1, NPRL3, OAS1, OR1B1, OR51F1, P2RX5, PDE4DIP, PIGN, POLDIP2, POLR3B, PYGL, RNF212, RXFP2, SCAPER, SELPLG, SIGLEC12, SLC37A4, SRA1, TAP2, TGIF1, TIGD6, TLDC2, TMEM107, TMEM216, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZAN