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WTSIi046-B

HPSI0214i-wibj_1

iPSC line

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General#

Cell Line

hPSCreg name WTSIi046-B
Alternative name(s)
HPSI0214i-wibj_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi046-A
(HPSI0214i-wibj_2)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi046-B
BioSamples SAMEA2627566
HipSci HPSI0214i-wibj_1
Cellosaurus CVCL_AE64
Wikidata Q54890554

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 55-59
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398911
HipSci HPSI-wibj

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 55-59
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
38.539 1.525 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0214i-wibj_1
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266670
mpileup variant calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266860
BWA alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266764
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1279063
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1274907
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1274915
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1882670
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447893
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1279085
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266944
Splice-aware STAR alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266982
Abundances of transcripts
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127551
Genotyping array calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127316
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1279081
Raw sequencing reads
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
collagen 6-related myopathy (COL6A2)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
congenital fibrosis of extraocular muscles (KIF21A)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
Fanconi anemia complementation group A (FANCA)
Hermansky-Pudlak syndrome 4 (HPS4)
Leigh syndrome (HIBCH)
Lynch syndrome (PMS1)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
Mowat-Wilson syndrome (ZEB2)
mucopolysaccharidosis type 4A (GALNS)
multiple acyl-CoA dehydrogenase deficiency (ETFB)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 7 (DNAH11)
primary ciliary dyskinesia 15 (CCDC40)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
X-linked complex neurodevelopmental disorder (ZNF711)
Other WGS-derived genes
AGAP6, AGL, ALDH3A2, ANO7, AURKC, BMP4, CASP12, CASP8, CHST15, CLDN16, COLQ, CST3, D2HGDH, DEFB126, DSC3, FBXO7, FUT2, FZD6, GALNT3, GPRC6A, HSD17B13, IDO2, IL17RC, ITGB2, KCNJ16, KISS1, KRT18, LAMA5, LPAR6, LPL, MESP1, MICA, MROH8, MTTP, NDUFB9, NFU1, OAS1, OR1B1, P2RX5, PDE4DIP, PIGN, PITX2, POLDIP2, PTCHD3, PYGL, RXFP2, SIGLEC12, SLC37A4, SPATA7, TAP2, TBP, TGIF1, TIGD6, TLR5, TMEM107, TMEM216, TMPRSS6, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, XDH, ZNF527