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WTSIi042-B

HPSI1013i-jufd_3

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi042-B
Alternative name(s)
HPSI1013i-jufd_3
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi042-B
BioSamples SAMEA2399461
HipSci HPSI1013i-jufd_3
Cellosaurus CVCL_AH69
ECACC 77650091, 66540182
CLO CLO_0101006
Wikidata Q54891560

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 45-49
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398326
HipSci HPSI-jufd

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 45-49
Collected in 2013
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Passage method EDTA
Surface coating Vitronectin
O2 concentration 20
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-4
Yes
SSEA-1
No
TRA 1-60
Yes
Pluripotency Score Novelty Score Link to microarray data
33.357 1.375 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1013i-jufd_3
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 4
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266838
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266744
mpileup variant calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266934
BWA alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1243467
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203392
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203424
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127417
Imputed and phased genotypes
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127652
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861342
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448224
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266968
Splice-aware STAR alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267067
Abundances of transcripts
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1203464
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203440
Raw sequencing reads
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Charcot-Marie-Tooth disease (FIG4, HINT1)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
Fanconi anemia complementation group A (FANCA)
Huntington disease (HTT)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, SLC26A5)
PHARC syndrome (ABHD12)
platelet-type bleeding disorder 11 (GP6)
primary ciliary dyskinesia 7 (DNAH11)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23)
Other WGS-derived genes
A2M, AGL, ANAPC1, ATXN3, AURKC, BMP4, CARD8, CASP12, CASP8, CATSPER2, CLDN16, CNOT1, COLQ, CYP21A2, CYP2D6, D2HGDH, DNAAF1, DNAJA4, DSC3, EFCAB3, FANCM, FLG, FUT2, FZD6, GALNT3, GLYCTK, HSD17B13, IL12RB1, IRF5, ITGB2, KCNJ16, KISS1, LAMA5, MAPT, MESP1, MICA, MROH8, MTTP, NDUFB9, NFU1, NOTCH2, NPRL3, OAS1, OR1B1, OTOR, PDE4DIP, PIGN, PITX2, POLDIP2, PRKRA, PTCHD3, PYGL, RXFP2, SCAPER, SLC37A4, SRA1, SYNE2, TAP2, TDG, TIGD6, TLR5, TMEM216, TMPRSS15, TMPRSS6, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZAN