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WTSIi038-A

HPSI1113i-ieki_3

iPSC line

Stock not immediately available - enquire for details
Purchases have been temporarily disabled, as we are transitioning sales and distribution.

If you are interested in purchasing this cell line, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi038-A
Alternative name(s)
HPSI1113i-ieki_3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi038-B
(HPSI1113i-ieki_2)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi038-A
BioSamples SAMEA2445787
HipSci HPSI1113i-ieki_3
Cellosaurus CVCL_AH94
CLO CLO_0100999
Wikidata Q54891636

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 55-59
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398488
HipSci HPSI-ieki

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 55-59
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
58.842 0.95 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1113i-ieki_3
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267054
Abundances of transcripts
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266962
Splice-aware STAR alignment
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127330
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203396
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1243471
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203428
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1203468
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861339
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266921
BWA alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266731
mpileup variant calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266825
Imputed and phased genotypes
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127565
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448177
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203444
Raw sequencing reads
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
autosomal recessive limb-girdle muscular dystrophy (SGCA)
Charcot-Marie-Tooth disease (FIG4, HINT1)
Cohen syndrome (VPS13B)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (NPPA)
familial ovarian cancer (GEN1)
hereditary breast carcinoma (GEN1)
Leigh syndrome (HIBCH)
Lynch syndrome (PMS1)
methylcobalamin deficiency type cblG (MTR)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
nephronophthisis 4 (NPHP4)
neuronal ceroid lipofuscinosis (MFSD8)
nonsyndromic genetic hearing loss (CDH23)
PHARC syndrome (ABHD12)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
Other WGS-derived genes
A2M, AGL, ALDH3A2, AMPD1, ATXN3, AURKC, BMP4, CARD8, CASP12, CATSPER2, CHIT1, CHST15, CLDN16, CNOT1, COLQ, CYP21A2, CYP2D6, CYP2F1, D2HGDH, DNAAF1, DSC3, DSE, FANCM, FLG, FUT2, FZD6, GALNT3, GDPD4, GLYCTK, GPR161, HSD17B13, IL12RB1, IRF5, ITGB2, KCNJ16, KCTD18, KISS1, LAMA5, MROH8, NDUFB9, NFU1, NPRL3, OAS1, OPRM1, OR51F1, OR52B4, P2RX5, PCK2, PDE4DIP, PIGN, PITX2, POLDIP2, POLR3B, PTCHD3, PYGL, SIGLEC12, SLC37A4, SPATA7, SRA1, TDG, TGIF1, TIGD6, TMEM216, TMPRSS6, TMX3, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZAN