WTSIi030-B

HPSI0214i-pelm_1

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi030-B
Alternative name(s)
HPSI0214i-pelm_1
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi030-B
BioSamples SAMEA2678742
HipSci HPSI0214i-pelm_1
Cellosaurus CVCL_AE61
ECACC 77650089, 66540128
CLO CLO_0100994
Wikidata Q54890548

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 40-44
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398777
HipSci HPSI-pelm

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 40-44
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium Essential E8
Passage method EDTA
Surface coating Vitronectin
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Pluripotency Score Novelty Score Link to microarray data
31.43 1.386 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0214i-pelm_1
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127276
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123087
Splice-aware STAR alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914308
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947085
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ123020
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947077
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266978
Abundances of transcripts
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914344
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447865
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122954
mpileup variant calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127511
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861350
Raw sequencing reads
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
breast-ovarian cancer, familial, susceptibility to, 2 (BRCA2)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
collagen 6-related myopathy (COL6A2)
complex neurodevelopmental disorder (SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (NPPA)
factor XIII, b subunit, deficiency of (F13B)
familial ovarian cancer (GEN1)
Fanconi anemia complementation group D1 (BRCA2)
hereditary breast carcinoma (GEN1)
Hermansky-Pudlak syndrome 4 (HPS4)
Leigh syndrome (HIBCH)
methylcobalamin deficiency type cblG (MTR)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 7 (DNAH11)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
Other WGS-derived genes
AGAP1, AGL, ALDH3A2, ATXN3, AURKC, BMP4, CARD8, CASP12, CASP8, CHST15, CLDN16, CLDN2, COLQ, CYP21A2, CYP2F1, CYP4B1, D2HGDH, DEFB126, DSC3, DUSP6, ERCC6L2, FMO2, FUT2, FZD6, GALNT3, GDPD4, GLYCTK, GPRC6A, H6PD, HEY1, HLA-DRB5, HSD17B13, IL12RB1, ITGB2, KCNJ16, LAD1, LAMA5, MESP1, MMP3, MROH8, MTTP, NDUFB9, NFU1, NPRL3, NXF2, OAS1, OPRM1, OR1B1, OR51F1, OR52B4, P2RX5, PDE4DIP, PIEZO1, PIGN, PITX2, POLDIP2, POLR3B, PRKRA, PTCHD3, SELPLG, SIGLEC12, SLC37A4, SRA1, SYNE2, TBP, TGIF1, TIGD6, TMEM107, TMEM216, TMPRSS6, TOR1AIP1, TREH, TRPM1, WDR37, ZNF83, ZNF99