WTSIi029-A
HPSI1013i-garx_2
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
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hPSCreg Name | WTSIi029-A |
Alternative name(s) |
HPSI1013i-garx_2
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
WTSIi003-A (HPSI1213i-hehd_2) WTSIi005-A (HPSI1113i-podx_1) WTSIi007-A (HPSI1113i-dons_1) WTSIi009-A (HPSI0913i-diku_1) WTSIi011-A (HPSI0314i-qonc_2) WTSIi012-A (HPSI0314i-bubh_1) WTSIi015-A (HPSI0114i-rozh_5) WTSIi020-A (HPSI0114i-eipl_1) WTSIi021-A (HPSI1013i-wuye_2) WTSIi024-A (HPSI0114i-vabj_3) WTSIi026-A (HPSI0314i-hoik_1) WTSIi027-A (HPSI0913i-oapg_5) WTSIi028-A (HPSI1213i-babk_2) WTSIi030-A (HPSI0214i-pelm_3) WTSIi040-A (HPSI0314i-qaqx_1) WTSIi041-A (HPSI0114i-bezi_1) WTSIi015-B (HPSI0114i-rozh_4) WTSIi021-B (HPSI1013i-wuye_3) WTSIi044-A (HPSI0214i-eiwy_1) WTSIi045-A (HPSI0214i-heth_1) WTSIi005-B (HPSI1113i-podx_2) WTSIi046-A (HPSI0214i-wibj_2) WTSIi047-A (HPSI0814i-bokz_5) WTSIi038-B (HPSI1113i-ieki_2) WTSIi048-A (HPSI0214i-datg_2) WTSIi047-B (HPSI0814i-bokz_6) WTSIi049-A (HPSI0514i-wiii_2) WTSIi030-B (HPSI0214i-pelm_1) WTSIi049-B (HPSI0514i-wiii_3) WTSIi052-A (HPSI1114i-ziyn_6) WTSIi055-A (HPSI0513i-debk_9) WTSIi056-A (HPSI0513i-dipe_1) WTSIi057-A (HPSI0513i-giuf_3) WTSIi058-A (HPSI0513i-suzg_3) WTSIi060-A (HPSI0513i-veqz_6) WTSIi061-A (HPSI0513i-veve_2) WTSIi062-A (HPSI0513i-zaui_3) WTSIi066-A (HPSI0613i-febc_1) WTSIi067-A (HPSI0713i-foop_1) WTSIi067-B (HPSI0713i-foop_2) WTSIi071-A (HPSI0913i-lise_1) WTSIi066-B (HPSI0613i-febc_2) WTSIi012-B (HPSI0314i-bubh_3) WTSIi074-A (HPSI0114i-joxm_1) WTSIi017-B (HPSI0114i-lexy_1) WTSIi075-A (HPSI0114i-vass_1) WTSIi076-A (HPSI0214i-kehc_2) WTSIi077-A (HPSI0314i-cuhk_1) WTSIi011-B (HPSI0314i-qonc_1) WTSIi071-B (HPSI0913i-lise_3) |
Provider |
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Depositor | Wellcome Sanger Institute (WTSI) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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Derivation country | United Kingdom |
External Databases |
|
hPSCreg | WTSIi029-A |
BioSamples | SAMEA2398552 |
HipSci | HPSI1013i-garx_2 |
Cellosaurus | CVCL_AH63 |
ECACC | 77650138, 66540099 |
CLO | CLO_0100992 |
Wikidata | Q54891545 |
General Information |
|
Publications | View all related publications on hPSCreg (1) |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
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Sex | female |
Age of donor (at collection) | 50-54 |
Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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External Databases (Donor) |
|
BioSamples | SAMEA2398675 |
HipSci | HPSI-garx |
hIPSC Derivation#
General |
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Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
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Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
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Age of donor (at collection) | 50-54 |
Collected in | 2013 |
Source cell line vendor | Cambridge BioResource |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
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Other |
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Selection criteria for clones | Morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
Culture medium | Essential E8 |
Passage method | EDTA |
Surface coating | Vitronectin |
O2 concentration | 21 |
CO2 concentration | 5 |
Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
CO2 Concentration | 5 % |
Medium |
TeSR™ E8™
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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SOX2 |
Yes |
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NANOG |
Yes |
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SSEA-1 |
No |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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Pluripotency Score | Novelty Score | Link to microarray data |
30.142 | 1.39 | http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/ |
Report
HPSI-garx.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-garx.pluritest.novelty_score.20161010.png
novelty image
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
No
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Other Genotyping (Cell Line) |
|
Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266824
cnv
http://www.hipsci.org/lines/#/lines/HPSI1013i-garx_2 Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0 |
WGS-derived disease associations |
centronuclear myopathy (CCDC78)
Charcot-Marie-Tooth disease (FIG4)
classic homocystinuria (CBS)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (NPPA)
Fanconi anemia complementation group A (FANCA)
galactosemia (GALT)
hereditary nonpolyposis colon cancer (XRCC4)
hyperprolinemia type 1 (PRODH)
methylcobalamin deficiency type cblG (MTR)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
mucopolysaccharidosis type 4A (GALNS)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (LOXHD1, MCM2)
primary ciliary dyskinesia 7 (DNAH11)
PTEN hamartoma tumor syndrome (PTEN)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
X-linked complex neurodevelopmental disorder (SLC35A2, ZNF711)
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Other WGS-derived genes | A2M, ABCA10, ABCC12, AGAP1, AGL, AKR1E2, ALDH3A2, ATXN3, AURKC, BMP4, CARD8, CASP12, CASP8, CATSPER2, CERKL, CLDN16, COG2, CST3, CYP3A5, D2HGDH, DEFB126, DNAAF1, DNAH9, DSC3, DUSP6, DYDC2, ERCC6L2, FBXO7, FLG2, FUT2, GDPD4, GLYCTK, GPRC6A, GPRIN1, HSD17B13, IDO2, IL12RB1, KCNJ16, KRT18, LAMA5, LAMB4, MESP1, MICA, MROH8, MTTP, NDUFB9, NPRL3, OAS1, OR51F1, P2RX5, PDE4DIP, PIGN, POLDIP2, PTCHD3, PYGL, RNF212, RP1L1, RXFP2, SCAPER, SELPLG, SHOX, SIGLEC12, SLC37A4, SRA1, TAP2, TGIF1, TIGD6, TMEM216, TMX3, TOR1AIP1, TPPP2, TREH, VDR, WDR37, ZAN, ZNF107, ZNF141, ZNF233 |