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WTSIi026-A

HPSI0314i-hoik_1

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi026-A
Alternative name(s)
HPSI0314i-hoik_1
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi026-A
BioSamples SAMEA2698315
HipSci HPSI0314i-hoik_1
Cellosaurus CVCL_AE82
ECACC 77650129, 66540096
CLO CLO_0100989
Wikidata Q54890615

General Information

Publications View all related publications on hPSCreg (2)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 40-44
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2399961
HipSci HPSI-hoik

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 40-44
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium Essential E8
Passage method EDTA
Surface coating Vitronectin
O2 concentration 20
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Pluripotency Score Novelty Score Link to microarray data
25.252 1.538 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0314i-hoik_1
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 0
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861337
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266887
BWA alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266697
mpileup variant calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1203456
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203384
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203416
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448006
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203408
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127265
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1243459
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266791
Imputed and phased genotypes
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127500
Genotyping array calls
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
familial ovarian cancer (GEN1)
Fanconi anemia complementation group A (FANCA)
galactosemia (GALT)
hereditary breast carcinoma (GEN1)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A, TMPRSS3)
primary ciliary dyskinesia 7 (DNAH11)
primary ciliary dyskinesia 14 (CCDC39)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
Other WGS-derived genes
AGAP6, ALDH3A2, ANAPC1, ATXN3, AURKC, BMP4, CASP12, CHST15, CLDN16, COLQ, CST3, CYP21A2, CYP2F1, D2HGDH, DEFB126, DNAAF1, DSC3, DSE, DUSP6, ERCC6L2, FUT2, FZD6, GALNT3, GDPD4, GLYCTK, GPRIN1, HEY1, ITGB2, KCNJ16, KCNMB3, LAMA5, LPAR6, MAPT, MED15, MROH8, MTTP, NDUFB9, NFU1, OAS1, OPRM1, OR1B1, OR52B4, P2RX5, P2RX7, PDE4DIP, PIGN, POLDIP2, PTCHD3, PYGL, RGPD4, RNF212, RXFP2, SIGLEC12, SLC37A4, SPATA7, SRA1, TIGD6, TLR5, TMEM107, TMEM216, TNRC18, TOR1AIP1, TPTE, TREH, TRPM1, VDR, WDR37, ZAN, ZNF233, ZNF83