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WTSIi019-A

HPSI0114i-iisa_3

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi019-A
Alternative name(s)
HPSI0114i-iisa_3
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi019-A
BioSamples SAMEA2536410
HipSci HPSI0114i-iisa_3
Cellosaurus CVCL_AE05
ECACC 77650082, 66540089
CLO CLO_0100976
Wikidata Q54890426

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 55-59
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398646
HipSci HPSI-iisa

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 55-59
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium Essential E8
Passage method EDTA
Surface coating Vitronectin
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Pluripotency Score Novelty Score Link to microarray data
28.614 1.25 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0114i-iisa_3
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122952
mpileup variant calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ123018
Imputed and phased genotypes
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127378
Imputed and phased genotypes
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860938
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127613
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266984
Abundances of transcripts
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR946969
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR780166
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860458
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860554
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860650
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR847020
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR946994
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859882
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861034
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861130
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123085
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860746
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860842
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447908
GATK haplotype calls
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861226
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860362
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859978
Raw sequencing reads
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
collagen 6-related myopathy (COL6A2)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
familial ovarian cancer (NF1)
hereditary nonpolyposis colon cancer (XRCC4)
Hermansky-Pudlak syndrome 4 (HPS4)
Leigh syndrome (HIBCH)
Lynch syndrome (PMS1)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
neurofibromatosis type 1 (NF1)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
primary ciliary dyskinesia 7 (DNAH11)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
Usher syndrome type 1 (CDH23, MYO7A)
Other WGS-derived genes
A2M, AGAP1, AGAP6, AGL, ALDH3A2, AURKC, BMP4, CARD8, CASP12, CATSPER2, CHST15, CLEC7A, CNOT1, COLQ, D2HGDH, DEFB126, DNAAF1, DSC3, DUSP6, FBXO7, FCGR2A, FMO2, FUT2, GALNT3, GDPD4, GLYCTK, GPRC6A, GPRIN1, H6PD, HEY1, IRF5, ITGB2, KAT2A, KCNJ16, LAMA5, LPL, MAPT, MED15, MROH8, NDUFB9, NOTCH2, NXF2, OAS1, OPRM1, OR1B1, P2RX5, PAX6, PDE4DIP, PIGN, PITX2, POLDIP2, RXFP2, SCAPER, SIGLEC12, SLC37A4, SRA1, SULT1C3, SYNE2, TAP2, TGIF1, TMEM216, TMPRSS6, TNRC18, TOR1AIP1, TREH, TRPM1, UNC93A, VDR, WDR37