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WTSIi018-B
HPSI0114i-kolf_2
iPSC line
The cell line was withdrawn.
General#
Cell Line |
|
| hPSCreg name | WTSIi018-B |
| Alternative name(s) |
HPSI0114i-kolf_2
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
WTSIi018-A (HPSI0114i-kolf_3) |
Provider |
|
| Depositor | Wellcome Sanger Institute (WTSI) |
| Distributors |
EBiSC
|
| Derivation country | United Kingdom |
External Databases |
|
| hPSCreg | WTSIi018-B |
| BioSamples | SAMEA2547615 |
| HipSci | HPSI0114i-kolf_2 |
| Cellosaurus | CVCL_AE29 |
| Wikidata | Q54890431 |
General Information |
|
| Publications | View all related publications on hPSCreg (15) |
| This EBiSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
| Subclones |
|
Donor Information#
General Donor Information |
|
| Sex | male |
| Age of donor (at collection) | 55-59 |
| Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA2398402 |
| HipSci | HPSI-kolf |
hIPSC Derivation#
General |
|
| Source cell type |
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
|
| Source cell origin |
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
|
| Age of donor (at collection) | 55-59 |
| Collected in | 2014 |
| Source cell line vendor | Cambridge BioResource |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| CO2 Concentration | 5 % |
| Medium |
TeSR™ E8™
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|
||||
| SOX2 |
Yes |
|
||||
| NANOG |
Yes |
|
| Pluripotency Score | Novelty Score | Link to microarray data |
| 48.039 | 0.876 | http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/ |
Report
HPSI-kolf.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-kolf.pluritest.novelty_score.20161010.png
novelty image
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266771
cnv
http://www.hipsci.org/lines/#/lines/HPSI0114i-kolf_2 Number of regions different from primary tissue: 1; Length of differences from primary tissue: 0 |
| WGS-derived disease associations |
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Bernard-Soulier syndrome (GP1BA)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
Hermansky-Pudlak syndrome 4 (HPS4)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (ABCC8, PAX4)
mucopolysaccharidosis type 4A (GALNS)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
Noonan syndrome (A2ML1)
PHARC syndrome (ABHD12)
phenylketonuria (PAH)
platelet-type von Willebrand disease (GP1BA)
primary ciliary dyskinesia 7 (DNAH11)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
|
| Other WGS-derived genes | AGL, ANAPC1, ARMS2, ATP13A5, AURKC, CASP12, CHST15, CLEC7A, CYP21A2, CYP2D6, CYP4B1, DEFB126, DSC3, FANCM, FBXO7, FCN3, FZD6, GALNT3, GDPD4, GLYCTK, GPR151, GPR161, GPRIN1, H6PD, IL12RB1, ITGB2, KCNJ16, LAMA5, MICA, MROH8, NDUFB9, NFU1, OAS1, OPRM1, OR1B1, P2RX5, PDE4DIP, PIGN, POLDIP2, PTCHD3, RNF212, SIGLEC12, SLC37A4, SRA1, SYNE2, TBP, TIGD6, TMEM107, TMEM216, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZAN, ZNF141 |