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WTSIi017-B

HPSI0114i-lexy_1

iPSC line

Not-for-profit fee: £1400 per vial
Immediately available for distribution*
*Once all legal and processing details completed
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name WTSIi017-B
Alternative name(s)
HPSI0114i-lexy_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi017-A
(HPSI0114i-lexy_2)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi017-B
BioSamples SAMEA2536416
HipSci HPSI0114i-lexy_1
Cellosaurus CVCL_AE31
Wikidata Q54890434

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 60-64
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2399225
HipSci HPSI-lexy

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 60-64
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Passage method EDTA
Surface coating Vitronectin
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Pluripotency Score Novelty Score Link to microarray data
24.53 1.221 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0114i-lexy_1
Number of regions different from primary tissue: 4; Length of differences from primary tissue: 129
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122967
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123034
Splice-aware STAR alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR847026
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR947015
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860830
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860734
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860638
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127538
Genotyping array calls
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861022
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860062
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448081
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR780172
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860446
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860542
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127303
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR946990
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860926
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267030
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122901
mpileup variant calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861310
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861214
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861118
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859966
Raw sequencing reads
WGS-derived disease associations
Angelman syndrome (UBE3A)
Charcot-Marie-Tooth disease (FIG4, HINT1)
Cohen syndrome (VPS13B)
collagen 6-related myopathy (COL6A2)
complex neurodevelopmental disorder (SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
epilepsy (EFHC1)
Fanconi anemia complementation group A (FANCA)
Lynch syndrome (PMS1)
mitochondrial disease (NDUFS6)
mucopolysaccharidosis type 4A (GALNS)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 5 (HYDIN)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
Other WGS-derived genes
ADAM33, AGAP6, AGL, AHNAK, ALDH3A2, ATXN3, AURKC, CARD8, CASP12, CATSPER2, CHIT1, CHST15, CLCNKB, CNOT1, COLQ, CYP21A2, CYP2D6, CYP2F1, CYP4B1, D2HGDH, DEFB126, DNAAF1, DSC3, FANCM, FBXO7, FCGR2A, GALNT3, GDPD4, HSD17B13, IL12RB1, IRF5, ITGB2, KCNJ16, KISS1, MICA, MROH8, MTTP, NBAS, NDUFB9, NFU1, OAS1, OR1B1, OR51F1, OTOR, P2RX5, PDE4DIP, PIGN, PNPLA1, POLDIP2, PRKRA, RAD52, RNF212, RP1L1, SERPINA10, SIGLEC12, SLC34A1, SLC37A4, SRA1, TDG, TGIF1, TIGD6, TMEM216, TOR1AIP1, TREH, TRPM1, VDR, VRK1, WDR37, ZNF141, ZNF844