WTSIi013-A
HPSI0214i-kucg_2
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
| hPSCreg Name | WTSIi013-A |
| Alternative name(s) |
HPSI0214i-kucg_2
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | |
Provider |
|
| Depositor | Wellcome Sanger Institute (WTSI) |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
| Derivation country | United Kingdom |
External Databases |
|
| hPSCreg | WTSIi013-A |
| BioSamples | SAMEA2645814 |
| HipSci | HPSI0214i-kucg_2 |
| Cellosaurus | CVCL_AE60 |
| ECACC | 77650065, 66540083 |
| CLO | CLO_0100966 |
| Wikidata | Q54890547 |
General Information |
|
| Publications | View all related publications on hPSCreg (4) |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
|
| Sex | male |
| Age of donor (at collection) | 65-69 |
| Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA2397923 |
| HipSci | HPSI-kucg |
hIPSC Derivation#
General |
|
| Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
| Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
| Age of donor (at collection) | 65-69 |
| Collected in | 2014 |
| Source cell line vendor | Cambridge BioResource |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
| Culture medium | Essential E8 |
| Passage method | EDTA |
| Surface coating | Vitronectin |
| O2 concentration | 20 |
| CO2 concentration | 5 |
| Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| CO2 Concentration | 5 % |
| Medium |
TeSR™ E8™
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|
|
|||
| SOX2 |
Yes |
|
||||
| NANOG |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| SSEA-1 |
No |
|
||||
| TRA 1-60 |
Yes |
|
| Pluripotency Score | Novelty Score | Link to microarray data |
| 36.792 | 1.631 | http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/ |
Report
HPSI-kucg.pluritest.pluripotency_score.20170926.png
pluripotency image
HPSI-kucg.pluritest.novelty_score.20170926.png
novelty image
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Sterility |
|
| Inoculation for microbiological growth | No Contaminants Detected |
| Mycoplasma | Not Detected |
| Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122983
cnv
http://www.hipsci.org/lines/#/lines/HPSI0214i-kucg_2 Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/ Text file with probe intensities |
| WGS-derived disease associations |
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Brugada syndrome 1 (CACNB2)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
collagen 6-related myopathy (COL6A2)
complex neurodevelopmental disorder (SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
Fanconi anemia complementation group A (FANCA)
galactosemia (GALT)
Huntington disease (HTT)
hypertrophic cardiomyopathy (CACNB2)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
mucopolysaccharidosis type 1 (IDUA)
mucopolysaccharidosis type 4A (GALNS)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
PHARC syndrome (ABHD12)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
short QT syndrome (CACNB2)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
Usher syndrome type 1 (CDH23, MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
|
| Other WGS-derived genes | AGAP1, AGAP6, AGL, ALDH3A2, ANAPC1, ATXN3, AURKC, BMP4, C1orf141, CARD8, CASP12, CATSPER2, CHIT1, CHST15, COLQ, COQ2, CST3, CYP21A2, CYP2D6, D2HGDH, DSC3, ERCC6L2, FCGR2A, FMO2, FUT2, GAL3ST2, GALNT3, GDPD4, GPRIN1, H6PD, IDO2, ITGB2, KCNJ16, LPL, MESP1, MPDZ, MROH8, NDUFB9, NPRL3, NRP2, P2RX5, PDE4DIP, PIGN, POLDIP2, POLR3B, PTCHD3, PYGL, RNF212, SCAPER, SIGLEC12, SLC37A4, SPATA7, SPTBN5, SRA1, SYNE2, TGIF1, TGM4, TMC3, TMEM107, TMEM175, TMEM216, TNRC18, TOR1AIP1, TREH, TRPM1, VRK1, WDR37, ZAN, ZNF233, ZNF527 |