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WTSIi012-A

HPSI0314i-bubh_1

iPSC line

Stock not immediately available - enquire for details
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name WTSIi012-A
Alternative name(s)
HPSI0314i-bubh_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi012-A
BioSamples SAMEA2675459
HipSci HPSI0314i-bubh_1
Cellosaurus CVCL_AE77
Wikidata Q54890608

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 35-39
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2434172
HipSci HPSI-bubh

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 35-39
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium Essential E8
Passage method EDTA
Surface coating Vitronectin
O2 concentration 20
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Pluripotency Score Novelty Score Link to microarray data
5.978 1.699 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0314i-bubh_1
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914320
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123055
Splice-aware STAR alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947071
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448125
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122988
Imputed and phased genotypes
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127541
Genotyping array calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127306
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267044
Abundances of transcripts
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914284
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861314
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122922
mpileup variant calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947079
Raw sequencing reads
WGS-derived disease associations
autosomal recessive nonsyndromic hearing loss 63 (LRTOMT)
Charcot-Marie-Tooth disease (FIG4, HINT1)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
congenital fibrosis of extraocular muscles (KIF21A)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
Fanconi anemia complementation group A (FANCA)
Lynch syndrome (PMS1)
mitochondrial disease (NDUFS6)
monogenic diabetes (ABCC8, PAX4)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
primary ciliary dyskinesia 5 (HYDIN)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
Other WGS-derived genes
A2M, AGL, AKR1E2, ALDH3A2, AURKC, BMP4, CARD8, CASP12, CHRM2, CLEC7A, CYP21A2, D2HGDH, DNAAF1, DSC3, FBXO7, FCGR2A, FZD6, GDPD4, GPRIN1, HLA-DRB5, HSD17B13, IDO2, ITGB2, KCNJ16, KLHL3, LAMA5, MICA, MROH8, MTTP, NBPF1, NDUFB9, NFU1, NOTCH2, OAS1, OPRM1, OR1B1, OR51F1, P2RX5, PDE4DIP, PIEZO1, PIGN, POLDIP2, POLR3B, PRKRA, PTCHD3, RXFP2, SCAPER, SIGLEC12, SLC37A4, STAG2, TGIF1, TIGD6, TMEM107, TMEM216, TMPRSS6, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZAN, ZNF208, ZNF83