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UNEWi026-B

SF116 clone 2

iPSC line

Stock not immediately available - enquire for details
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name UNEWi026-B
Alternative name(s)
SF116 clone 2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UNEWi026-C
(SF116 clone K)
Donor's gene variants:
CFH
Donor diseases:
type 2 diabetes mellitus
age-related macular degeneration
UNEWi026-A
(SF116 clone 1)
Donor's gene variants:
CFH
Donor diseases:
type 2 diabetes mellitus
age-related macular degeneration
STBCi110-A
(SFC116-03-01)
Donor's gene variants:
CFH
Donor diseases:
type 2 diabetes mellitus
age-related macular degeneration
UNEWi024-A
(F180-1)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi024-B
(F180-2)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi024-C
(F180-3)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi022-A
(F181 5.8)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi022-B
(F181 18.2)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi022-C
(F181 25.7)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
STBCi107-A
(SFC104-03-01)
Donor diseases:
type 2 diabetes mellitus
STBCi126-A
(SFC105-03-01)
Donor diseases:
type 2 diabetes mellitus
STBCi098-A
(SFC048-07-14)
Donor diseases:
type 2 diabetes mellitus
STBCi098-B
(SFC048-07-18)
Donor diseases:
type 2 diabetes mellitus
STBCi100-A
(SFC117-03-01)
Donor diseases:
type 2 diabetes mellitus
STBCi098-C
(SFC048-07-17)
Donor diseases:
type 2 diabetes mellitus
STBCi122-A
(SFC115-03-01)
Donor diseases:
type 2 diabetes mellitus
STBCi127-A
(SFC107-03-01)
Donor diseases:
type 2 diabetes mellitus
UNEWi004-A
(PRPF31 SH)
Donor's gene variants:
PRPF31
Donor diseases:
Retinitis pigmentosa
UNEWi005-A
(PRPF31 RH)
Donor's gene variants:
PRPF31
Donor diseases:
Retinitis pigmentosa
UKBi003-A
(iLB-MJD1-32m-r9, LB-32-r9)
Donor's gene variants:
ATXN3, ATXN3
Donor diseases:
Spinocerebellar ataxia type 3
UKBi008-A
(iLB-MJD4-34m-r1, LB-34-1)
Donor's gene variants:
ATXN3, ATXN3
Donor diseases:
Spinocerebellar ataxia type 3
UKBi001-B
Donor's gene variants:
ATXN3
Donor diseases:
Spinocerebellar ataxia type 3
UNEWi001-A
(UNEW001Ai)
Donor's gene variants:
PRPF31
Donor diseases:
Retinitis pigmentosa
UNEWi002-A
(UNEW002Ai, PRPF31 AW)
Donor's gene variants:
PRPF31, PRPF31
Donor diseases:
Retinitis pigmentosa
UKBi007-A
(LB-33-5, iLB-MJD3-33f-r5)
Donor's gene variants:
ATXN3, ATXN3
Donor diseases:
Spinocerebellar ataxia type 3
UNEWi027-A
(F116)
Donor's gene variants:
PRPF31
Donor diseases:
Retinitis pigmentosa

Provider

Depositor University of Newcastle (UNEW)
Owner Institute of Genetic Medicine
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg UNEWi026-B
BioSamples SAMEA4567590
Cellosaurus CVCL_IT98
Wikidata Q54991227

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 60-64

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Sporadic typical T2D
Synonyms
  • NIDDM
  • non-insulin-dependent diabetes mellitus
  • type 2 diabetes
  • type II diabetes mellitus
show more synonyms
Genetic variants
Donor is at high risk of developing age-related macular degeneration but has not been diagnosed with AMD at time of biopsy.
The donor is a carrier of a disease-associated mutation and not affected.
Synonyms
  • AMD
  • ARMD
  • Senile macular degeneration
  • Senile macular retinal degeneration
  • age related Maculopathies
  • age related maculopathy
  • age-related macular degeneration
  • macular degeneration, age-related
  • age related macular degeneration
show more synonyms
Genetic variants
CFH (target)
1q31.3
NM_000186.3:c.1204C>T
NP_000177.2:p.His402Tyr NC_000001.11:g.19669010
Homozygous
SCV000038294.5

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4582266

hIPSC Derivation#

General

Source cell type
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 60-64
Passage number reprogrammed P4

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Selection criteria for clones Based on morphology, growth rate and expression of SSEA4 and NANOG
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 20
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
Alpha FetoProtein
Yes
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
SMA
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
TUJ-1
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No clinically significant imbalance was detected
Passage number: P11
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)