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iPSC line

Not-for-profit fee: £1400 per vial
Immediately available for distribution*
*Once all legal and processing details completed
Timepoint: 48 hours post-passage
Magnification: x4
Timepoint: 48 hours post-passage
Magnification: x10
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.


Cell Line

hPSCreg name UNEWi018-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines


Depositor University of Newcastle (UNEW)
Derivation country United Kingdom

External Databases

hPSCreg UNEWi018-A
BioSamples SAMEA3142165
Cellosaurus CVCL_II94
Wikidata Q54991159

General Information

This EBiSC line can be used for:
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 10-14
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Disease associated phenotypes
  • pancytopenia and hypocellular bone marrow
Family history No
Is the medical history available upon request? No
Is clinical information available? Idiopathic severe aplastic anaemia. Responded to rabbit Lymphoglobulin but then relapsed. Now in partial recovery following treatment with lymphocyte immune globulin

External Databases (Donor)

BioSamples SAMEA3142155

hIPSC Derivation#


Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 10-14

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming


Derived under xeno-free conditions
Derived under GMP?
Available as clinical grade?

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
O2 Concentration 95 %
CO2 Concentration 5 %
Medium mTeSR™ 1


Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
TRA 1-60
Differentiation Potency
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative


Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation


Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
trisomy chromosome 3
Karyotyping method: CytoSNP

Other Genotyping (Cell Line)