Please take our EBiSC Customer Survey.

UNEWi011-A

UNEW011Ai

iPSC line

The cell line was withdrawn.

General#

Cell Line

hPSCreg Name UNEWi011-A
Alternative name(s)
UNEW011Ai
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor University of Newcastle (UNEW)
Distributors
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg UNEWi011-A
BioSamples SAMEA2629624
Cellosaurus CVCL_VF32
Wikidata Q54991156
CLO CLO_0103597

General Information

* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 1-4
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Cernunnos-XLF deficiency
XLF Deficiency
The donor is affected.
Stage
Severe
Synonyms
  • Cernunnos XLFD
  • NHEJ1 deficiency
  • Cernunnos deficiency
  • Combined immunodeficiency-microcephaly-growth retardation-sensitivity to ionizing radiation syndrome
show more synonyms
Non-disease associated phenotypes
  • primary immunodeficiency, severe growth retardation, bird-like face, recurrent perianal abscess, pancytopenia, and polydactyly
Family history NO

External Databases (Donor)

BioSamples SAMEA2629494

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 1-4

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 95 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

No characterisation data could be found.

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unknown

Other Genotyping (Cell Line)