UNEWi002-A

UNEW002Ai, PRPF31 AW

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name UNEWi002-A
Alternative name(s)
UNEW002Ai, PRPF31 AW
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UNEWi001-A
(UNEW001Ai)
Donor's gene variants:
PRPF31
Donor diseases:
Retinitis pigmentosa
UNEWi027-A
(F116)
Donor's gene variants:
PRPF31
Donor diseases:
Retinitis pigmentosa
UNEWi004-A
(PRPF31 SH)
Donor's gene variants:
PRPF31
Donor diseases:
Retinitis pigmentosa
UNEWi005-A
(PRPF31 RH)
Donor's gene variants:
PRPF31
Donor diseases:
Retinitis pigmentosa
ESi077-A
(CABi001-A, PRPF31-MiPS4F3)
Donor's gene variants:
PRPF31, PRPF31
Donor diseases:
Retinitis Pigmentosa
UNEWi024-A
(F180-1)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi022-B
(F181 18.2)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi022-C
(F181 25.7)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi026-A
(SF116 clone 1)
Donor's gene variants:
CFH
Donor diseases:
type 2 diabetes mellitus
age-related macular degeneration
UNEWi024-B
(F180-2)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi024-C
(F180-3)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi026-B
(SF116 clone 2)
Donor's gene variants:
CFH
Donor diseases:
type 2 diabetes mellitus
age-related macular degeneration
UNEWi026-C
(SF116 clone K)
Donor's gene variants:
CFH
Donor diseases:
type 2 diabetes mellitus
age-related macular degeneration
UNEWi022-A
(F181 5.8)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi003-A
(PRPF31-HD)
Donor diseases:
Retinitis pigmentosa

Provider

Depositor University of Newcastle (UNEW)
Owner Institute of Genetic Medicine
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Institute of Genetic Medicine

External Databases

hPSCreg UNEWi002-A
BioSamples SAMEA2629491
Cellosaurus CVCL_9S38
ECACC 66540040
CLO CLO_0101556
Wikidata Q54991152

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 50-54

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Retinitis pigmentosa
The donor is a carrier of a disease-associated mutation and affected.
Genetic variants
PRPF31 (target)
19q13.42
PRPF31: c.522_527+10del
Disease associated phenotypes
  • Retinitis Pigmentosa

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

External Databases (Donor)

BioSamples SAMEA2629633

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 50-54

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other

Selection criteria for clones Stem cell morphology and growth speed
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
Unknown

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes