UKKi036-B
NP0143-B, NP0143-15
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
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hPSCreg Name | UKKi036-B |
Alternative name(s) |
NP0143-B, NP0143-15
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
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Depositor | Klinikum der Universität zu Köln (UKK) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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Derivation country | Germany |
External Databases |
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hPSCreg | UKKi036-B |
BioSamples | SAMEA104615863 |
ECACC | 66540899 |
Cellosaurus | CVCL_VF46 |
CLO | CLO_0100896 |
Wikidata | Q54990546 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
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Donor Information#
General Donor Information |
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Sex | female |
Age of donor (at collection) | 20-24 |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
Yes
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA104615904 |
hIPSC Derivation#
General |
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Source cell type |
peripheral blood mononuclear cellA leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
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Age of donor (at collection) | 20-24 |
Collected in | 2016 |
Passage number reprogrammed | 0 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Type of used vector free reprogramming factor(s) |
None
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Other |
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Selection criteria for clones | morphology |
Derived under xeno-free conditions |
Yes |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Vitronectin | ||||||
Feeder cells |
No |
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Passage method |
Enzyme-free cell dissociation
EDTA
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O2 Concentration | 20 % | ||||||
CO2 Concentration | 5 % | ||||||
Medium |
Other medium:
Base medium: StemFlex
Main protein source: Albumine Serum concentration: 0 % Supplements
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Differentiation Potency
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
no larger chromosomal aberrations- Chr.20 early mosaicism of whole chromosome visible (20%)
Passage number: 12
Karyotyping method:
Molecular karyotyping by SNP array
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
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