UKKi006-A
D4-cB, M4C4, NP0053-5
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg Name | UKKi006-A |
Alternative name(s) |
D4-cB, M4C4, NP0053-5
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | |
Provider |
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Depositor | Klinikum der Universität zu Köln (UKK) |
Owner | Institute for Neurophysiology, Medical Faculty |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Institute for Neurophysiology, Medical Faculty
Scottish Centre for Regenerative Medicine
|
Derivation country | Germany |
External Databases |
|
hPSCreg | UKKi006-A |
BioSamples | SAMEA2825851 |
Cellosaurus | CVCL_9S59 |
ECACC | 66540018 |
CLO | CLO_0100871 |
Wikidata | Q54990421 |
General Information |
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Publications | |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
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Donor Information#
General Donor Information |
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Sex | female |
Age of donor (at collection) | 60-64 |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Disease associated phenotypes |
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Family history | No |
Is the medical history available upon request? | No |
Is clinical information available? | No |
Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
|
BioSamples | SAMEA2825874 |
hIPSC Derivation#
General |
|
Source cell type |
mesenchymal stem cell of the bone marrowA mesenchymal stem cell that is part of the bone marrow.
|
Source cell origin |
bone marrowThe soft tissue that fills the cavities of bones.
|
Source cell type (free text) | Bone marrow-derived mesenchymal stromal cells (MSCs) |
Age of donor (at collection) | 60-64 |
Collected in | 2010 |
Passage number reprogrammed | P4 |
Reprogramming method |
|
Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
No |
Reprogramming vectors silenced? |
Yes |
Methods used |
RT-PCR
|
Notes on reprogramming vector silencing | Retrovirally encoded reprogramming factors OSKM are not expressed in iPSCs as determined by RT-PCR |
Files and images showing reprogramming vector expressed or silenced | |
Vector map | |
Vector free reprogramming |
|
Type of used vector free reprogramming factor(s) |
None
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Other |
|
Selection criteria for clones | human ESC morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
Culture medium | Essential E8 |
Passage method | EDTA |
Surface coating | Vitronectin |
O2 concentration | 21 |
CO2 concentration | 5 |
Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-4 |
Yes |
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TRA 1-80 |
Yes |
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TRA1-80 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Not done |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
Deletion of the short arm and duplikation of the long arm of Chr20 were detected.
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
|
Is there genome-wide genotyping or functional data available? |
Yes
Methylation profiling
|