UKKi006-A
D4-cB, M4C4, NP0053-5
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
Timepoint: 48 hours post-passage
Magnification: 4x
Magnification: 4x
Timepoint: 48 hours post-passage
Magnification: 10x
Magnification: 10x
Timepoint: Confluence
Magnification: 4x
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
Magnification: 10x
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
| hPSCreg Name | UKKi006-A |
| Alternative name(s) |
D4-cB, M4C4, NP0053-5
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | |
Provider |
|
| Depositor | Klinikum der Universität zu Köln (UKK) |
| Owner | Institute for Neurophysiology, Medical Faculty |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Institute for Neurophysiology, Medical Faculty
Scottish Centre for Regenerative Medicine
|
| Derivation country | Germany |
External Databases |
|
| hPSCreg | UKKi006-A |
| BioSamples | SAMEA2825851 |
| Cellosaurus | CVCL_9S59 |
| ECACC | 66540018 |
| CLO | CLO_0100871 |
| Wikidata | Q54990421 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
|
Donor Information#
General Donor Information |
|
| Sex | female |
| Age of donor (at collection) | 60-64 |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes |
|
| Family history | No |
| Is the medical history available upon request? | No |
| Is clinical information available? | No |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA2825874 |
hIPSC Derivation#
General |
|
| Source cell type |
mesenchymal stem cell of the bone marrowA mesenchymal stem cell that is part of the bone marrow.
|
| Source cell origin |
bone marrowThe soft tissue that fills the cavities of bones.
|
| Source cell type (free text) | Bone marrow-derived mesenchymal stromal cells (MSCs) |
| Age of donor (at collection) | 60-64 |
| Collected in | 2010 |
| Passage number reprogrammed | P4 |
Reprogramming method |
|
| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Genes | |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
No |
| Reprogramming vectors silenced? |
Yes |
| Methods used |
RT-PCR
|
| Notes on reprogramming vector silencing | Retrovirally encoded reprogramming factors OSKM are not expressed in iPSCs as determined by RT-PCR |
| Files and images showing reprogramming vector expressed or silenced | |
| Vector map | |
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
None
|
Other |
|
| Selection criteria for clones | human ESC morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
| Culture medium | Essential E8 |
| Passage method | EDTA |
| Surface coating | Vitronectin |
| O2 concentration | 21 |
| CO2 concentration | 5 |
| Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
|
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| SSEA-4 |
Yes |
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| TRA 1-80 |
Yes |
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| TRA1-80 |
Yes |
|
Morphology pictures
brightfield image of NP0053-5 iPSC colonies, day 2 post-thaw; 10x objective
brightfield image of NP0053-5 iPSC colony, day 2 post-thaw; 32x objective
Differentiation Potency
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Not done |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Sterility |
|
| Inoculation for microbiological growth | No Contaminants Detected |
| Mycoplasma | Not Detected |
| Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
Deletion of the short arm and duplikation of the long arm of Chr20 were detected.
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Methylation profiling
|