UCLi002-A
HHItC9D-V34, DN19
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg Name | UCLi002-A |
Alternative name(s) |
HHItC9D-V34, DN19
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
|
Depositor | University College London (UCL) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
External Databases |
|
hPSCreg | UCLi002-A |
BioSamples | SAMEA3174461 |
Cellosaurus | CVCL_9S55 |
ECACC | 66540029 |
CLO | CLO_0101582 |
Wikidata | Q54989667 |
General Information |
|
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
|
Donor Information#
General Donor Information |
|
Sex | male |
Age of donor (at collection) | 55-59 |
Phenotype and Disease related information (Donor) |
|
Diseases | A disease was diagnosed.
|
Karyotyping (Donor) |
|
Has the donor karyotype been analysed? |
Yes
|
External Databases (Donor) |
|
BioSamples | SAMEA3174486 |
hIPSC Derivation#
General |
|
Source cell type |
fibroblast of dermis |
Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
Age of donor (at collection) | 55-59 |
Reprogramming method |
|
Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
Unknown |
Absence of reprogramming vector(s)? |
Unknown |
Reprogramming vectors silenced? | |
Vector free reprogramming |
|
Other |
|
Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
Culture medium | Essential E8 |
Passage method | EDTA |
Surface coating | Matrigel / Geltrex |
O2 concentration | 21 |
CO2 concentration | 5 |
Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
CO2 Concentration | 5 % |
Medium |
Essential 8™
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
|
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SSEA-4 |
Yes |
|
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TRA 1-60 |
Yes |
|
||||
SSEA-1 |
No |
|
Differentiation Potency
In vitro spontaneous differentiation
Marker | Expressed |
SOX17 |
Yes |
FOXA2 |
Yes |
GSC |
No |
GATA6 |
Yes |
CXCR4 |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
HAND1 |
Yes |
VIMENTIN |
Yes |
BMP4 |
Yes |
GATA4 |
Yes |
PITX1 |
Yes |
DECORIN |
Yes |
PECAM1 (CD31) |
Yes |
PDGFRA |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
PAX6 |
Yes |
Sox1 |
Yes |
BUTUBULIN |
Yes |
NEUROD1 |
Yes |
HES5 |
Yes |
FOXG1 |
No |
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
46, XY
Passage number: 49
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |