The EBiSC team is working hard to implement improvements in how EBiSC operates. Due to some short-term disruption, please get in touch via Contact@EBiSC.org if the cells you would like to access are currently listed as unavailable or you are ordering from outside of Europe.

UCLi002-A

HHItC9D-V34, DN19

iPSC line

Stock not immediately available - enquire for details
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name UCLi002-A
Alternative name(s)
HHItC9D-V34, DN19
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UCLi001-A
(HHItC9S-V19)
Donor's gene variants:
C9orf72
Donor diseases:
Frontotemporal dementia
UCLi004-C
(RCi172, RCFB60c2)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Amyotrophic lateral sclerosis
Frontotemporal dementia
UCLi004-B
(RCFB60c7, RCi177)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Amyotrophic lateral sclerosis
Frontotemporal dementia
UCLi004-A
(RCi173, RCFB60c6)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Amyotrophic lateral sclerosis
Frontotemporal dementia
UCLi003-A
(TSM(exon10+16)V97)
Donor's gene variants:
MAPT
Donor diseases:
Frontotemporal dementia
LUBi001-B
(PGRN-8310, RCFB58 c4.4, PGRN8310, RCi194)
Donor's gene variants:
GRN, GRN, GRN, GRN
Donor diseases:
Frontotemporal dementia
LUBi001-C
(PGRN-8310, RCFB58 c3.7, RCi200, PGRN8310)
Donor's gene variants:
GRN, GRN, GRN, GRN
Donor diseases:
Frontotemporal dementia
UKBi001-B
Donor's gene variants:
ATXN3
Donor diseases:
Spinocerebellar ataxia type 3
UKBi003-A
(iLB-MJD1-32m-r9, LB-32-r9)
Donor's gene variants:
ATXN3, ATXN3
Donor diseases:
Spinocerebellar ataxia type 3
UKBi008-A
(iLB-MJD4-34m-r1, LB-34-1)
Donor's gene variants:
ATXN3, ATXN3
Donor diseases:
Spinocerebellar ataxia type 3
UOXFi003-A
(MK088-1)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UKBi007-A
(LB-33-5, iLB-MJD3-33f-r5)
Donor's gene variants:
ATXN3, ATXN3
Donor diseases:
Spinocerebellar ataxia type 3

Provider

Depositor University College London (UCL)
Distributors
EBiSC

External Databases

hPSCreg UCLi002-A
BioSamples SAMEA3174461
Cellosaurus CVCL_9S55
Wikidata Q54989667

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 55-59

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • FTD
Genetic variants
C9orf72 (target)
9p21.2
The identified mutation is Hexanucleotide repeat expansion in C9ORF72 at 9p21.2.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

External Databases (Donor)

BioSamples SAMEA3174486

hIPSC Derivation#

General

Source cell type
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 55-59

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium Essential E8
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
GSC
No
GATA6
Yes
CXCR4
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
HAND1
Yes
VIMENTIN
Yes
BMP4
Yes
GATA4
Yes
PITX1
Yes
DECORIN
Yes
PECAM1 (CD31)
Yes
PDGFRA
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
Sox1
Yes
BUTUBULIN
Yes
NEUROD1
Yes
HES5
Yes
FOXG1
No

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 49
Karyotyping method: G-Banding

Other Genotyping (Cell Line)