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STBCi166-A

SFC169-03-01

iPSC line

The cell line was withdrawn.

General#

Cell Line
hPSCreg Name STBCi166-A
Alternative name(s)
SFC169-03-01
Cell line type Human induced pluripotent stem cell (hiPSC)
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Provider
Depositor StemBANCC (STBC)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases
hPSCreg STBCi166-A
BioSamples SAMEA104616256
Cellosaurus CVCL_RE36
Wikidata Q54970345
CLO CLO_0104850

General Information
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information
Sex male
Age of donor (at collection) 60-64

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.

External Databases (Donor)
BioSamples SAMEA104616257

hIPSC Derivation#

General
Source cell type
fibroblast
fibroblast in the OLS
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 60-64

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

No characterisation data could be found.

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)