STBCi137-A

SFC076-03-01

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name STBCi137-A
Alternative name(s)
SFC076-03-01
Cell line type Human induced pluripotent stem cell (hiPSC)
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Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
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Donor's gene variants:
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Donor diseases:
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Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg STBCi137-A
BioSamples SAMEA104616198
ECACC 66540957
Cellosaurus CVCL_RE07
Wikidata Q54970296
CLO CLO_0104821

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 55-59

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA104616199

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 55-59

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes
Morphology pictures
Morphology 24h and 5 days after thawing

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Four small abnormalities detected (See red dots in image)
Passage number: 10
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)