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RCi003-B

CIP11A-9, RCi117

iPSC line

In Stock
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line
hPSCreg Name RCi003-B
Alternative name(s)
CIP11A-9, RCi117
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider
Depositor Pfizer Limited - Pfizer (PFIZ)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases
hPSCreg RCi003-B
BioSamples SAMEA3106143
Cellosaurus CVCL_9S46
ECACC 66540052
CLO CLO_0101615
Wikidata Q54949503

General Information
* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information
Sex female

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
pain agnosia
pain agnosia in the OLS
The donor is affected.
Synonyms
  • pain agnosia
  • analgesia
  • agnosia for pain
Disease associated phenotypes
  • Chronic Insensitivity to Pain
  • SCN11a

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA3105960

hIPSC Derivation#

General
Source cell type
erythroblast
erythroblast in the OLS
A nucleated precursor of an erythrocyte that lacks hematopoietic lineage markers.
Source cell type (free text) Blood - PBMC erythroblasts
Passage number reprogrammed NA

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Latest released batch
Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility
Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
18 cells out of 20 normal, 1 cell 47,XXX, one cell 45,XX,-17
RCi117_P16_10Nov2014.pdf
Passage number: P16
Karyotyping method: G-Banding

Other Genotyping (Cell Line)
Is there genome-wide genotyping or functional data available?
Yes