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PFIZi029-A

B222c4

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
Timepoint: 48hr post thaw
Magnification: x4
Timepoint: 48hr post thaw
Magnification: x10
Timepoint: Confluency
Magnification: x4
Timepoint: Confluency
Magnification: x10
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name PFIZi029-A
Alternative name(s)
B222c4
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Pfizer Limited - Pfizer (PFIZ)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg PFIZi029-A
BioSamples SAMEA104491453
ECACC 66540640
Cellosaurus CVCL_VE77
CLO CLO_0101956
Wikidata Q54947283

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 5-9

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
myoclonic epilepsy (primary disease)
Myoclonic encephalopathy
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Myoclonic Epilepsy, Infantile, Benign
  • Epilepsy, Myoclonic, Infantile, Benign
  • Symptomatic Myoclonic Epilepsy
  • Myoclonic Seizure Disorder
  • Severe Infantile Myoclonic Epilepsy
  • Myoclonic Epilepsy, Severe Infantile
  • Benign Infantile Myoclonic Epilepsy
  • Epilepsy, Myoclonic, Infantile, Severe
  • Myoclonic Absence EpilepsyMyoclonic Astatic Epilepsy
  • Early Childhood Epilepsy, Myoclonic
  • Myoclonic Epilepsy
  • Epilepsy, Early Childhood, Myoclonic
  • Epilepsy, Myoclonic, Infantile
  • Severe Myoclonic Epilepsy, Infantile
  • Myoclonic Epilepsy, Infantile, Severe
  • Epilepsy, Myoclonic, Early Childhood
  • Myoclonic Epilepsy, Benign InfantileMyoclonic Epilepsy, Early Childhood
  • Encephalopathy, Myoclonic
  • Myoclonic Encephalopathy
  • Severe Myoclonic Epilepsy Of Infancy
  • Myoclonic Epilepsy, Infantile
  • Myoclonic Epilepsy, Severe, Of Infancy
  • Infantile Severe Myoclonic Epilepsy
  • Early Childhood, Myoclonic Epilepsy
  • Epilepsy, Myoclonus
  • Idiopathic Myoclonic Epilepsy
  • Cryptogenic Myoclonic Epilepsy
show more synonyms
Genetic variants
CHD2 (target)
15q26.1
NM_001271.3:c.4636C>T
NP_001262.3:p.Arg1546Ter
SCV000573177.3
c4636 C>T in exon 36 of the CHD2 gene (NM_001271.3)

External Databases (Donor)

BioSamples SAMEA104491454

hIPSC Derivation#

General

Source cell type
peripheral blood mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 5-9

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR™1
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
No
GATA6
Yes
CXCR4
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Vimentin
Yes
NCAM
Yes
MIXL1
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
PAX6
Yes
NeuroD1
Yes
HES5
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX modal karyotype in 20 cells showed a normal female chromosome and complement banding pattern
Passage number: 19
Karyotyping method: G-Banding

Other Genotyping (Cell Line)