PFIZi028-A
B212c7
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg Name | PFIZi028-A |
Alternative name(s) |
B212c7
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
UKKi027-A (NP0101-A, NP0101-3D) UKKi027-C (NP0101-C, NP0101-10H) UKKi028-A (NP0115-A, NP0115-4) UKKi028-B (NP0115-B) UKKi028-C (NP0115-C) UKKi036-A (NP0143-A, NP0143-5) UKKi026-C (NP0114-5E) UKKi035-B (NP0139-B, NP0139-6C) Donor's gene variants: MYBPC3, MYBPC3 Donor diseases: Hypertrophic cardiomyopathy UKKi037-C (NP0144-C, NP0144-41) UKKi026-A (NP0114-1A) UKKi006-A (D4-cB, M4C4, NP0053-5) UKKi007-A (NP0014-6, UKK007Ai) Donor's gene variants: RYR2, RYR2 Donor diseases: Catecholaminergic polymorphic ventricular tachycardia UKKi007-B (NP0014-5) Donor's gene variants: RYR2 Donor diseases: Catecholaminergic polymorphic ventricular tachycardia UNEWi002-A (UNEW002Ai, PRPF31 AW) Donor's gene variants: PRPF31, PRPF31 Donor diseases: Retinitis pigmentosa UKBi005-A (iLB-C-31f-r1, LB-31-1) UKBi007-A (LB-33-5, iLB-MJD3-33f-r5) Donor's gene variants: ATXN3, ATXN3 Donor diseases: Spinocerebellar ataxia type 3 EDi001-A (AST22, AST23, SAMEA3319992) Donor's gene variants: SNCA, SNCA, SNCA Donor diseases: Parkinson disease EDi002-A (NAS2) WTSIi003-A (HPSI1213i-hehd_2) WTSIi005-A (HPSI1113i-podx_1) |
Provider |
|
Depositor | Pfizer Limited - Pfizer (PFIZ) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
External Databases |
|
hPSCreg | PFIZi028-A |
BioSamples | SAMEA104491452 |
ECACC | 66540639 |
Cellosaurus | CVCL_VE76 |
CLO | CLO_0101713 |
Wikidata | Q54947282 |
General Information |
|
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
|
Donor Information#
General Donor Information |
|
Sex | female |
Age of donor (at collection) | 5-9 |
Phenotype and Disease related information (Donor) |
|
Diseases | A disease was diagnosed.
|
External Databases (Donor) |
|
BioSamples | SAMEA104491451 |
hIPSC Derivation#
General |
|
Source cell type |
peripheral blood mononuclear cellA leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
|
Age of donor (at collection) | 5-9 |
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
RT-PCR
|
Vector free reprogramming |
|
Other |
|
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
Culture medium | mTeSR™1 |
Passage method | EDTA |
Surface coating | matrigel / Geltrex |
O2 concentration | 21 |
CO2 concentration | 5 |
Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
|
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TRA 1-60 |
Yes |
|
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SSEA-4 |
Yes |
|
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SSEA-1 |
No |
|
Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
46, XX; Modal karyotype in 20 cells showed a normal female chromosome complement and banding pattern
Passage number: 12
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |