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PFIZi024-A

B218c6

iPSC line

Not-for-profit fee: £1400 per vial
Immediately available for distribution*
*Once all legal and processing details completed
Timepoint: 48hr post thaw
Magnification: x4
Timepoint: 48hr post thaw
Magnification: x10
Timepoint: Confluency
Magnification: x4
Timepoint: Confluency
Magnification: x10
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line
hPSCreg name PFIZi024-A
Alternative name(s)
B218c6
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
PFIZi025-A
(B220c16)
Donor's gene variants:
KCNC1
Donor diseases:
epilepsy

Provider
Depositor Pfizer Limited - Pfizer (PFIZ)
Distributors
EBiSC

External Databases
hPSCreg PFIZi024-A
BioSamples SAMEA104236754
Cellosaurus CVCL_RG01
Wikidata Q54947279

General Information
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information
Sex male
Age of donor (at collection) 30-34

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Epilepsy
Mioclonic epilepsy
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • epilepsy
  • Epileptic attack, NOS
  • Epilepsy, unspecified, with intractable epilepsy
  • Generalized seizure (finding)
  • Epileptic seizures
  • Other forms of epilepsy (disorder)
  • Epileptic seizures, NOS
  • [X]Other epilepsy (disorder)
  • Seizure disorder (disorder)
  • Generalized fit
  • Epilepsy (disorder)
  • Epileptic fits
  • Seizure disorder
  • Other forms of epilepsy
  • Epilectic attack, NOS
  • Other forms of epilepsy, with intractable epilepsy
  • Epileptic fits, NOS
  • Epileptic disorder
  • Epilepsy, NOS
  • EPILEP NEC W/O INTR EPIL
  • EPILEPSY NOS W INTR EPIL
  • Epileptic Seizure
  • Epileptic attack
  • Epileptic seizure (finding)
  • seizure disorder
  • [X]Other epilepsy
  • EP - Epilepsy
  • EPILEPSY NEC W INTR EPIL
  • Other forms of epilepsy NOS
  • Other forms of epilepsy and recurrent seizures
  • Epileptic convulsions, NOS
  • Epileptic disorder, NOS
  • Epilepsy, unspecified, without mention of intractable epilepsy
  • Epilepsy, unspecified
  • Epilepsy and recurrent seizures
  • Epilepsy NOS (disorder)
  • EPILEP NOS W/O INTR EPIL
  • Epileptic fit
  • Epilepsy NOS
  • Other forms of epilepsy, without mention of intractable epilepsy
  • Epileptic
  • Generalized seizure
  • Generalized convulsion
  • Generalised seizure
  • Generalised convulsion
  • Other forms of epilepsy NOS (disorder)
  • EF - Epileptic fit
  • Generalised fit
  • Epileptic convulsions
show more synonyms
Genetic variants
GABRA1 (target)
5q34
NM_000806.5:c.440G>A
NP_000797.2:p.Arg147Gln
Heterozygous
rs1376907797
p.Arg147Gln (R147Q) (CGG>CAG): c.440 G>A in exon 6 of the GABRA1 gene (NM_000806.5)

Karyotyping (Donor)
Has the donor karyotype been analysed?
No

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)
BioSamples SAMEA104236755

hIPSC Derivation#

General
Source cell type
Erythroblast
A nucleated precursor of an erythrocyte that lacks hematopoietic lineage markers.
Synonyms
  • normoblast
Age of donor (at collection) 30-34

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming
Type of used vector free reprogramming factor(s)
None

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch
Culture medium mTeSR™1
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
CXCR4
Yes
GATA6
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
VIM
Yes
NCAM1
Yes
MIXL1
No
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
NeuroD1
Yes
HES5
Yes

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility
Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 11
Karyotyping method: G-Banding

Other Genotyping (Cell Line)