The EBiSC team is working hard to implement improvements in how EBiSC operates. Due to some short-term disruption, please get in touch via Contact@EBiSC.org if the cells you would like to access are currently listed as unavailable or you are ordering from outside of Europe.

PFIZi021-A

OD001-s7

iPSC line

Stock not immediately available - enquire for details
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
To receive the Certificate of Analysis, please contact us.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name PFIZi021-A
Alternative name(s)
OD001-s7
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
PFIZi017-A
(BC142c7, RCi209)
Donor's gene variants:
SCN1A, SCN1A
Donor diseases:
Dravet syndrome
PFIZi019-A
(RCi202, BC145c6)
Donor's gene variants:
SCN1A, SCN1A
Donor diseases:
Dravet syndrome
PFIZi014-A
(OD002-s7)
Donor's gene variants:
SCN1A
Donor diseases:
Dravet syndrome
PFIZi009-A
(RCi188)
Donor's gene variants:
SCN1A
Donor diseases:
Dravet syndrome
PFIZi018-A
(BC143c8, RCi207)
Donor's gene variants:
SCN1A, SCN1A
Donor diseases:
Dravet syndrome
PFIZi015-A
(OD003-s5)
Donor's gene variants:
SCN1A
Donor diseases:
Dravet syndrome
PFIZi016-A
(OD005-s9)
Donor's gene variants:
SCN1A
Donor diseases:
Dravet syndrome

Provider

Depositor Pfizer Limited - Pfizer (PFIZ)
Distributors
EBiSC

External Databases

hPSCreg PFIZi021-A
BioSamples SAMEA4563388
Cellosaurus CVCL_RF99
Wikidata Q54947276

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 10-14

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • SMEI
  • Severe myoclonus epilepsy of infancy
  • Severe myoclonic epilepsy of infancy
Genetic variants
SCN1A (target)
2q24.3
NM_001165963.1:c.5162C>G
SCV000090965.1
p.T1721K, c.5162 C>A De novo
Disease associated phenotypes
  • GTC seizure
  • Developemental delay
  • Normal station and wide based, shuffled gait

External Databases (Donor)

BioSamples SAMEA4563387

hIPSC Derivation#

General

Source cell type
A nucleated precursor of an erythrocyte that lacks hematopoietic lineage markers.
Synonyms
  • normoblast
Age of donor (at collection) 10-14

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
Self-renewal
Negative
Endoderm
Unknown
Mesoderm
Unknown
Ectoderm score
Unknown
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Scorecard
Mesoderm
Ont Id: UBERON_0000926
Scorecard
Ectoderm
Ont Id: UBERON_0000924
Scorecard

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
NO autosomal or sex chromosome aneuploidies were detected
Passage number: 21
Karyotyping method: Karyolite BoBs

Other Genotyping (Cell Line)