If the cells you would like to access are currently listed as unavailable
or you are ordering from outside of Europe please get in touch via
Contact@EBiSC.org.
ESi008-A
KiPS4F-8
iPSC line
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg name | ESi008-A |
Alternative name(s) |
KiPS4F-8
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | No similar lines found. |
Provider |
|
Depositor | Spanish Stem Cell Bank (ES) |
Owner | Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB) |
Distributors |
Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB)
EBiSC
|
Derivation country | Spain |
External Databases |
|
hPSCreg | ESi008-A |
BioSamples | SAMEA3303318 |
Cellosaurus | CVCL_C998 |
Wikidata | Q54899939 |
General Information |
|
Publications | |
This EBiSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
Donor Information#
General Donor Information |
|
Sex | male |
Ethnicity | caucasian |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
Family history | no |
Is the medical history available upon request? | no |
Is clinical information available? | no |
Other Genotyping (Donor) |
|
Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
Other cell lines of this donor |
|
External Databases (Donor) |
|
BioSamples | SAMEA3303305 |
hIPSC Derivation#
General |
|
Source cell type |
Keratinocyte from foreskin.
|
Source cell origin |
A retractable double-layered fold of skin and mucous membrane that covers the glans penis and protects the urinary meatus when the penis is not erect.
Synonyms
|
Collected in | 2008 |
Reprogramming method |
|
Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
Unknown |
Reprogramming vectors silenced? |
Yes |
Methods used |
RT-PCR
|
Vector free reprogramming |
|
Other |
|
Selection criteria for clones | morphological |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
Culture medium | mTeSR |
Passage method | EDTA |
Surface coating | Matrigel / Geltrex |
O2 concentration | 21 |
CO2 concentration | 5 |
Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Gelatin | |||||||||
Feeder cells |
Human Foreskin fibroblasts Cellfinder Ont Id: CELDA_000001419 |
|||||||||
Passage method | Mechanically | |||||||||
O2 Concentration | 21 % | |||||||||
CO2 Concentration | 5 % | |||||||||
Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
|
||||
NANOG |
Yes |
|
||||
POU5F1 (OCT-4) |
Yes |
|
||||
SSEA-3 |
Yes |
|
||||
SSEA-4 |
Yes |
|
||||
TRA 1-60 |
Yes |
|
||||
TRA 1-81 |
Yes |
|
||||
ZFP42 (REX-1) |
Yes |
|
||||
SOX2 |
Yes |
|
Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
Marker | Expressed |
GATA4 |
Yes |
ASA |
Yes |
Pax7 |
Yes |
SOX9 |
Yes |
In vivo teratoma
In vitro spontaneous differentiation
Marker | Expressed |
GFAP |
Yes |
Tuj1 |
Yes |
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
46XY
|
Other Genotyping (Cell Line) |
|
Is there genome-wide genotyping or functional data available? |
Yes
|