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ESi008-A
KiPS4F-8
iPSC line
Timepoint: Confluence
Magnification: 4x
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
Magnification: 10x
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
| hPSCreg name | ESi008-A |
| Alternative name(s) |
KiPS4F-8
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
Provider |
|
| Depositor | Spanish Stem Cell Bank (ES) |
| Owner | Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB) |
| Distributors |
Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB)
EBiSC
|
| Derivation country | Spain |
External Databases |
|
| hPSCreg | ESi008-A |
| BioSamples | SAMEA3303318 |
| Cellosaurus | CVCL_C998 |
| Wikidata | Q54899939 |
General Information |
|
| Publications | |
| This EBiSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
Donor Information#
General Donor Information |
|
| Sex | male |
| Ethnicity | caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | no |
| Is the medical history available upon request? | no |
| Is clinical information available? | no |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| Other cell lines of this donor |
|
External Databases (Donor) |
|
| BioSamples | SAMEA3303305 |
hIPSC Derivation#
General |
|
| Source cell type |
An epithelial cell of stratified squamous tissues, including skin, oral mucosa (Hiroshima et al., 2011), and esophagus (Whelan et al., 2018), that produces keratin proteins and secretes antimicrobial peptides to form a resilient barrier against environmental damage, dehydration, pathogens, and microbial invasion. It undergoes successive stages of differentiation marked by changes in keratin expression, supporting tissue integrity, wound repair, and contributing to immune defense.
|
| Source cell origin |
A retractable double-layered fold of skin and mucous membrane that covers the glans penis and protects the urinary meatus when the penis is not erect.
Synonyms
|
| Collected in | 2008 |
Reprogramming method |
|
| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Genes | |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? |
Yes |
| Methods used |
RT-PCR
|
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | morphological |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
| Culture medium | mTeSR |
| Passage method | EDTA |
| Surface coating | Matrigel / Geltrex |
| O2 concentration | 21 |
| CO2 concentration | 5 |
| Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Gelatin | |||||||||
| Feeder cells |
Human Foreskin fibroblasts Cellfinder Ont Id: CELDA_000001419 |
|||||||||
| Passage method | Mechanically | |||||||||
| O2 Concentration | 21 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
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| NANOG |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
||||
| SSEA-3 |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
||||
| ZFP42 (REX-1) |
Yes |
|
||||
| SOX2 |
Yes |
|
Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
| Marker | Expressed |
| GATA4 |
Yes |
| ASA |
Yes |
| Pax7 |
Yes |
| SOX9 |
Yes |
In vivo teratoma
In vitro spontaneous differentiation
| Marker | Expressed |
| GFAP |
Yes |
| Tuj1 |
Yes |
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XY
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
|