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ESi007-A
CBiPS1sv-4F-40
iPSC line
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
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hPSCreg name | ESi007-A |
Alternative name(s) |
CBiPS1sv-4F-40
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | No similar lines found. |
Notes | Please note that the correct synonym is "CBiPS1sv-4F-40", not "CBiPSsv-4F-40" as stated in PMID:30778227. [NM] |
Provider |
|
Depositor | Spanish Stem Cell Bank (ES) |
Owner | Inbiomed |
Distributors |
EBiSC
Inbiomed
|
Derivation country | Spain |
External Databases |
|
hPSCreg | ESi007-A |
BioSamples | SAMEA3303231 |
Cellosaurus | CVCL_V189 |
Wikidata | Q54808661 |
General Information |
|
Publications | View all related publications on hPSCreg (3) |
This EBiSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
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Donor Information#
General Donor Information |
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Sex | female |
Age of donor (at collection) | neonate |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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External Databases (Donor) |
|
BioSamples | SAMEA3303248 |
hIPSC Derivation#
General |
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Source cell type |
Any hematopoietic stem cell that is part of a umbilical cord blood.
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Source cell origin |
Blood that remains in the placenta and in the attached umbilical cord after childbirth[WP].; Cord blood is collected because it contains stem cells, which can be used to treat hematopoietic and genetic disorders.[WP]
Synonyms
|
Source cell type (free text) | Cord blood CD133+ |
Age of donor (at collection) | neonate |
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
RT-PCR
|
Vector free reprogramming |
|
Other |
|
Selection criteria for clones | morphological |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
Culture medium | mTeSR |
Passage method | EDTA |
Surface coating | Matrigel / Geltrex |
O2 concentration | 21 |
CO2 concentration | 5 |
Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Gelatin | ||||||||||||||||||
Feeder cells |
Mouse embryonic fibroblasts Cellfinder Ont Id: EFO_0004040 |
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Passage method | Mechanically | ||||||||||||||||||
CO2 Concentration | 5 % | ||||||||||||||||||
Medium |
Other medium:
Base medium: Knockout DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-3 |
Yes |
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TRA 1-60 |
Yes |
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ZFP42 (REX-1) |
Yes |
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SOX2 |
Yes |
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Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
Marker | Expressed |
SMA |
Yes |
MEOX |
Yes |
AAP |
Yes |
In vivo teratoma
In vitro spontaneous differentiation
Marker | Expressed |
Tuj1 |
Yes |
PAX6 |
Yes |
NeuroD3 |
Yes |
Sox1 |
Yes |
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
46,XX
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
|
Is there genome-wide genotyping or functional data available? |
Yes
|