We are currently making some changes to how orders are placed and because of this there will be a
short disruption period affecting order processing. Thank you for your patience while we work to
operate as smoothly as possible (November 14, 2023).
ESi005-A
cFA404-KiPS4F-1
iPSC line
Purchases have been temporarily disabled, as we are transitioning sales and distribution.
If you are interested in purchasing this cell line, please contact EBiSC directly. For more information about the current transition process see here.
If you are interested in purchasing this cell line, please contact EBiSC directly. For more information about the current transition process see here.
The cell line was withdrawn.
General#
Cell Line |
|
| hPSCreg Name | ESi005-A |
| Alternative name(s) |
cFA404-KiPS4F-1
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
Provider |
|
| Depositor | Spanish Stem Cell Bank (ES) |
| Owner | Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB) |
| Distributors |
Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB)
European Collection of Authenticated Cell Cultures (ECACC)
|
| Derivation country | Spain |
External Databases |
|
| hPSCreg | ESi005-A |
| BioSamples | SAMEA3303130 |
| Cellosaurus | CVCL_C192 |
| CLO | CLO_0102669 |
| Wikidata | Q54811421 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Unknown
|
Donor Relations |
|
| Other cell lines of this donor |
|
External Databases (Donor) |
|
| BioSamples | SAMEA3303136 |
hIPSC Derivation#
General |
|
| Source cell type |
keratinocyteAn epidermal cell which synthesizes keratin and undergoes a characteristic change as it moves upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
|
| Source cell type (free text) | The gene FANCD2 (3p25.3) was knocked out in the keratinocytes before iPS reprogramming as a genetic correction of Fanconi anemia. |
Reprogramming method |
|
| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Genes | |
| Is the used vector excisable? |
Unknown |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | Reporter gene EGFP |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Gelatin | |||||||||
| Feeder cells |
Human Foreskin Fibroblasts Cellfinder Ont Id: CELDA_000001419 |
|||||||||
| O2 Concentration | 20 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
||||
| NANOG |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
||||
| SSEA-3 |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
||||
| SOX2 |
Yes |
|
Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
| Marker | Expressed |
| alpha-actinin |
Yes |
In vivo teratoma
In vitro spontaneous differentiation
| Marker | Expressed |
| Tuj1 |
Yes |
| TH |
Yes |
| GFAP |
Yes |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 43
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|