ESi003-A
[CRTRd]FiPS3819-4F-2
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
Timepoint: Confluence
Magnification: 4x
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
Magnification: 10x
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
| hPSCreg Name | ESi003-A |
| Alternative name(s) |
[CRTRd]FiPS3819-4F-2
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
|
| Depositor | Spanish Stem Cell Bank (ES) |
| Owner | Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB) |
| Distributors |
Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB)
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
| Derivation country | Spain |
External Databases |
|
| hPSCreg | ESi003-A |
| BioSamples | SAMEA3303029 |
| Cellosaurus | CVCL_V154 |
| ECACC | 66540064 |
| CLO | CLO_0101545 |
| Wikidata | Q54832762 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information#
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
| Disease associated phenotypes |
|
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Unknown
|
External Databases (Donor) |
|
| BioSamples | SAMEA3303031 |
hIPSC Derivation#
General |
|
| Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
Reprogramming method |
|
| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Genes | |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? |
Yes |
| Methods used |
RT-PCR
|
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
None
|
Other |
|
| Selection criteria for clones | morphological |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
| Culture medium | mTeSR |
| Passage method | EDTA |
| Surface coating | Matrigel / Geltrex |
| O2 concentration | 21 |
| CO2 concentration | 5 |
| Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Gelatin | ||||||||||||||||||
| Feeder cells |
Human foreskin fibroblasts Cellfinder Ont Id: CELDA_000001419 |
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| Passage method | Mechanically | ||||||||||||||||||
| O2 Concentration | 21 % | ||||||||||||||||||
| CO2 Concentration | 5 % | ||||||||||||||||||
| Medium |
Other medium:
Base medium: Knockout Dulbecco's Modified Eagle's medium
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
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| NANOG |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
|
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
|
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| SOX2 |
Yes |
|
Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
| Marker | Expressed |
| GATA4 |
Yes |
| AAS |
Yes |
| ASMA |
Yes |
| ASA |
Yes |
In vivo teratoma
In vitro spontaneous differentiation
| Marker | Expressed |
| Tuj1 |
Yes |
| GFAP |
Yes |
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
|