ESi003-A
[CRTRd]FiPS3819-4F-2
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
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hPSCreg Name | ESi003-A |
Alternative name(s) |
[CRTRd]FiPS3819-4F-2
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
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Depositor | Spanish Stem Cell Bank (ES) |
Owner | Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB) |
Distributors |
Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB)
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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Derivation country | Spain |
External Databases |
|
hPSCreg | ESi003-A |
BioSamples | SAMEA3303029 |
Cellosaurus | CVCL_V154 |
ECACC | 66540064 |
CLO | CLO_0101545 |
Wikidata | Q54832762 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes |
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Disease associated phenotypes |
|
Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Unknown
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External Databases (Donor) |
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BioSamples | SAMEA3303031 |
hIPSC Derivation#
General |
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Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
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Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
Unknown |
Reprogramming vectors silenced? |
Yes |
Methods used |
RT-PCR
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Vector free reprogramming |
|
Type of used vector free reprogramming factor(s) |
None
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Other |
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Selection criteria for clones | morphological |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
Culture medium | mTeSR |
Passage method | EDTA |
Surface coating | Matrigel / Geltrex |
O2 concentration | 21 |
CO2 concentration | 5 |
Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Gelatin | ||||||||||||||||||
Feeder cells |
Human foreskin fibroblasts Cellfinder Ont Id: CELDA_000001419 |
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Passage method | Mechanically | ||||||||||||||||||
O2 Concentration | 21 % | ||||||||||||||||||
CO2 Concentration | 5 % | ||||||||||||||||||
Medium |
Other medium:
Base medium: Knockout Dulbecco's Modified Eagle's medium
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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SOX2 |
Yes |
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Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
Marker | Expressed |
GATA4 |
Yes |
AAS |
Yes |
ASMA |
Yes |
ASA |
Yes |
In vivo teratoma
In vitro spontaneous differentiation
Marker | Expressed |
Tuj1 |
Yes |
GFAP |
Yes |
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
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