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EPFLi005-A
hiPS45
iPSC line
The cell line was withdrawn.
General#
Cell Line |
|
| hPSCreg name | EPFLi005-A |
| Alternative name(s) |
hiPS45
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
Provider |
|
| Depositor | Swiss Federal Institute of Technology (EPFL) |
| Distributors |
|
External Databases |
|
| hPSCreg | EPFLi005-A |
| BioSamples | SAMEA104012167 |
| Cellosaurus | CVCL_VD38 |
| Wikidata | Q54832360 |
General Information |
|
| Publications | |
| This EBiSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
Donor Information#
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA104012328 |
hIPSC Derivation#
General |
|
| Source cell type (free text) | CD34+ Hematopoietic stem cell |
Reprogramming method |
|
| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Is the used vector excisable? |
Unknown |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Medium |
mTeSR™ 1
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|
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| SOX2 |
Yes |
|
|
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| NANOG |
Yes |
|
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| ZFP42 (REX-1) |
Yes |
|
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| LIN28A |
Yes |
|
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| DPPA2 |
Yes |
|
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Transcriptome Characterisation
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46,XY
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|