If the cells you would like to access are currently listed as unavailable
or you are ordering from outside of Europe please get in touch via
Contact@EBiSC.org.
EPFLi005-A
hiPS45
iPSC line
The cell line was withdrawn.
General#
Cell Line |
|
hPSCreg name | EPFLi005-A |
Alternative name(s) |
hiPS45
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | No similar lines found. |
Provider |
|
Depositor | Swiss Federal Institute of Technology (EPFL) |
Distributors |
|
External Databases |
|
hPSCreg | EPFLi005-A |
BioSamples | SAMEA104012167 |
Cellosaurus | CVCL_VD38 |
Wikidata | Q54832360 |
General Information |
|
Publications | |
This EBiSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
Donor Information#
General Donor Information |
|
Sex | male |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
BioSamples | SAMEA104012328 |
hIPSC Derivation#
General |
|
Source cell type (free text) | CD34+ Hematopoietic stem cell |
Reprogramming method |
|
Vector type | Integrating |
Vector | Virus (Lentivirus) |
Is the used vector excisable? |
Unknown |
Absence of reprogramming vector(s)? |
Unknown |
Reprogramming vectors silenced? | |
Vector free reprogramming |
|
Other |
|
Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Medium |
mTeSR™ 1
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
|
|
|||
SOX2 |
Yes |
|
|
|||
NANOG |
Yes |
|
||||
ZFP42 (REX-1) |
Yes |
|
||||
LIN28A |
Yes |
|
||||
DPPA2 |
Yes |
|
Score:
Marker | Present | Absent |
mCpG | ||
OCT4 |
Transcriptome Characterisation
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
46,XY
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |