EDi002-A
NAS2
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg Name | EDi002-A |
Alternative name(s) |
NAS2
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
EDi001-A (AST22, AST23, SAMEA3319992) Donor's gene variants: SNCA, SNCA, SNCA Donor diseases: Parkinson disease EDi001-A-2 (AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease EDi001-A-3 (AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease |
Provider |
|
Depositor | University of Edinburgh (ED) |
Owner | College of Medicine and Veterinary Medicine |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Roslin Cells (RC)
|
Derivation country | United States |
External Databases |
|
hPSCreg | EDi002-A |
BioSamples | SAMEA3333374 |
Cellosaurus | CVCL_AW98 |
ECACC | 66540059 |
CLO | CLO_0100622 |
Wikidata | Q54831976 |
General Information |
|
Publications | View all related publications on hPSCreg (1) |
* Is the cell line readily obtainable for third parties? |
No |
Donor Information#
General Donor Information |
|
Sex | female |
Age of donor (at collection) | 30-34 |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
Disease associated phenotypes |
|
Family history | Strong family history of Parkinson’s disease due to autosomal dominant inheritance of SNCA triplication. This volunteer did not inherit the mutation |
Donor Relations |
|
All cell lines of this donor's relatives |
Has mother:
|
External Databases (Donor) |
|
BioSamples | SAMEA3333373 |
hIPSC Derivation#
General |
|
Source cell type |
fibroblast of dermis |
Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
Age of donor (at collection) | 30-34 |
Reprogramming method |
|
Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
Unknown |
Absence of reprogramming vector(s)? |
Unknown |
Reprogramming vectors silenced? |
Yes |
Methods used |
RT-PCR
|
Vector free reprogramming |
|
Type of used vector free reprogramming factor(s) |
None
|
Other |
|
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
Culture medium | mTeSR |
Passage method | EDTA |
Surface coating | Matrigel / Geltrex |
O2 concentration | 21 |
CO2 concentration | 5 |
Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Laminin |
Feeder cells |
No |
Passage method |
Enzymatically
Accutase
|
O2 Concentration | 95 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
|
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SSEA-4 |
Yes |
|
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TRA 1-60 |
Yes |
|
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SSEA-1 |
No |
|
Differentiation Potency
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
46,XX
Passage number: 48
Karyotyping method:
Molecular karyotyping by SNP array
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM701392 |
Other Genotyping (Cell Line) |