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DRICUi009-A

ADLON44426UC

iPSC line

Re-stocking
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line
hPSCreg Name DRICUi009-A
Alternative name(s)
ADLON44426UC
Cell line type Human induced pluripotent stem cell (hiPSC)
Notes Further anonymized information about the donor including demographic and clinical data will be made available on the Dementia Platforms UK data portal: https://portal.dementiasplatform.uk/

Provider
Depositor UK Dementia Research Institute, Cardiff University (DRICU)
Owner UK Dementia Research Institute, Cardiff University (DRICU)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases
hPSCreg DRICUi009-A
ECACC 66770033
BioSamples SAMEA10291416
Cellosaurus CVCL_B3P7
Wikidata Q110432788
CLO CLO_0104333

General Information
* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: Disease research
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information
Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)
Has the donor karyotype been analysed?
Yes
Normal
IPMAR_ADLON44426UC_karyogram.pdf
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
Yes

External Databases (Donor)
BioSamples SAMEA10563839

hIPSC Derivation#

General
Source cell type
T-Lymphocyte
T-Lymphocyte in the OLS
A thymocyte-derived lymphocyte of immunological importance that is long-lived (months to years) and is responsible for cell-mediated immunity. T lymphocyte cells form rosettes with sheep erythrocytes and, in the presence of transforming agents (mitogens), differentiate and divide. These cells have the characteristic T3 surface marker and may be further divided into subsets according to function, such as helper, cytotoxic, etc.
Synonyms
  • T Cells
  • T Cell
  • T-Lymphocytes
  • Thymus-Dependent Lymphocytes
  • T cell
  • T-Lymphocyte
  • T-Cell
  • T lymphocyte
  • Thymus Derived Lymphocyte
  • T-Cells
show more synonyms
Source cell origin
Blood
Blood in the OLS
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
show more synonyms

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection Virus undetectable at passage 8
Files and images showing reprogramming vector expressed or silenced
IPMAR_DRICUi009A_Sendai_Cytotune_2_clearance.pdf

Vector free reprogramming

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 18 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Advanced DMEM/F12 (Gibco Thermo Fisher 12634010)
Main protein source: Albumine
Serum concentration: 0 %
Supplements
GlutaMAX (Gibco Thermo Fisher 35050061) 1 %
Heparin (Stem Cell Technologies 07980) 0.1 µg/ml
Ascorbic Acid-2-phosphate magnesium salt (Sigma A8960) 0.22 mM
HEPES 1M pH 7.4 (Gibco Thermo Fisher 15630080) 15 mM
FGF-2 basic 145aa (Miltenyi Biotech 130-093-842) 100 ng/ml
TGF-beta (Biolegend 781804) 2 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
–
NANOG
Yes
–
Morphology pictures
IPMAR_DRICUi009A_Morphology.pdf
Morphology of DRICUi009A on day 2 post thaw
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
C-X-C Chemokine Receptor Type 4
Yes
SRY-box transcription factor 17
Yes
Morphology
IPMAR_DRICUi009A_Endoderm .pdf
IPMAR_DRICUi009A_Endoderm
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
C-X-C Chemokine Receptor Type 4
Yes
BRACHYURY
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
Paired box 6
Yes
orthodenticle homeobox 2
Yes
Morphology
IPMAR_DRICUi009A_Ectoderm.pdf
IPMAR_DRICUi009A_Ectoderm

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
No gross abnormalities
IPMAR_DRICUi009-A_karyogram.pdf
Passage number: 13
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)