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CMDi001-A
01016
iPSC line
A CLIP contains information about an iPSC line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the iPSC line.
The EBiSC Access and Use Agreement must be completed along with an individual
Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org
for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
iPSC Line |
|
| hPSCreg name | CMDi001-A |
| Alternative name(s) |
01016
|
| iPSC line type | Human induced pluripotent stem cell (hiPSC) |
| Similar iPSC lines |
|
Provider |
|
| Depositor | CureCMD (CMD) |
| Owner | CureCMD (CMD) |
| Distributors |
EBiSC
FUJIFILM Cellular Dynamics, Inc. (CDI)
|
| Derivation country | United States |
External iPSC Databases |
|
| hPSCreg | CMDi001-A |
| BioSamples | SAMEA112655517 |
| Cellosaurus | CVCL_D0DG |
| Wikidata | Q123030943 |
General iPSC Information |
|
| Publications | View all related publications on hPSCreg (3) |
| This EBiSC iPSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
Donor Information#
General Donor Information |
|
| Sex | male |
| Age of donor (at collection) | 15-19 |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA112655518 |
iPSC Derivation#
General |
|
| Source cell type |
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
|
| Age of donor (at collection) | 15-19 |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Episomal |
| Is reprogramming vector detectable? |
No |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
iPSC Culture Conditions#
Latest released batch |
|
| Culture medium | mTeSR-1 |
| Passage method | EDTA |
| Surface coating | Matrigel |
| O2 concentration | 21 |
| CO2 concentration | 5 |
| Temperature | 37°C |
The following are the depositor culture conditions, they do not refer to any specific batch.
| Medium |
Essential 8™
|
iPSC Characterisation#
Analysis of undifferentiated iPSCs
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|||||
| NANOG |
Yes |
|||||
| Lin28 |
Yes |
|||||
| SSEA-1 |
Yes |
|||||
| SSEA-4 |
Yes |
|||||
| TRA 1-60 |
Yes |
|||||
| POU5F1 (OCT-4) |
Yes |
Undifferentiated cells were analyzed by proprietary assays as described in the attached publication. Pluripotency of established iPSC cultures were tested by qPCR on bulk samples using 48 genes including standard pluripotency genes (listed above). Samples were assigned a passing score after analysis with a non-supervised classifier based on an appropriate sample set. (Novak, T. 2015. Drug Discovery World pp. 47-53.)
Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Sterility |
|
| Inoculation for microbiological growth | No Contaminants Detected |
| Mycoplasma | Not Detected |
| Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (iPSC Line) |
|
| Has the iPSC line karyotype been analysed? |
Yes
Normal
|
Other Genotyping (iPSC Line) |
|