CHDIi051-A
#129c1, CHDI-90002199
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
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hPSCreg Name | CHDIi051-A |
Alternative name(s) |
#129c1, CHDI-90002199
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
WTSIi003-A (HPSI1213i-hehd_2) WTSIi005-A (HPSI1113i-podx_1) WTSIi007-A (HPSI1113i-dons_1) WTSIi009-A (HPSI0913i-diku_1) WTSIi011-A (HPSI0314i-qonc_2) WTSIi012-A (HPSI0314i-bubh_1) WTSIi015-A (HPSI0114i-rozh_5) WTSIi020-A (HPSI0114i-eipl_1) WTSIi021-A (HPSI1013i-wuye_2) WTSIi024-A (HPSI0114i-vabj_3) WTSIi026-A (HPSI0314i-hoik_1) WTSIi027-A (HPSI0913i-oapg_5) WTSIi028-A (HPSI1213i-babk_2) WTSIi029-A (HPSI1013i-garx_2) WTSIi030-A (HPSI0214i-pelm_3) WTSIi040-A (HPSI0314i-qaqx_1) WTSIi041-A (HPSI0114i-bezi_1) WTSIi015-B (HPSI0114i-rozh_4) WTSIi021-B (HPSI1013i-wuye_3) WTSIi044-A (HPSI0214i-eiwy_1) WTSIi045-A (HPSI0214i-heth_1) WTSIi005-B (HPSI1113i-podx_2) WTSIi046-A (HPSI0214i-wibj_2) WTSIi047-A (HPSI0814i-bokz_5) WTSIi038-B (HPSI1113i-ieki_2) WTSIi048-A (HPSI0214i-datg_2) WTSIi047-B (HPSI0814i-bokz_6) WTSIi049-A (HPSI0514i-wiii_2) WTSIi030-B (HPSI0214i-pelm_1) WTSIi049-B (HPSI0514i-wiii_3) WTSIi052-A (HPSI1114i-ziyn_6) WTSIi055-A (HPSI0513i-debk_9) WTSIi056-A (HPSI0513i-dipe_1) WTSIi057-A (HPSI0513i-giuf_3) WTSIi058-A (HPSI0513i-suzg_3) WTSIi060-A (HPSI0513i-veqz_6) WTSIi061-A (HPSI0513i-veve_2) WTSIi062-A (HPSI0513i-zaui_3) WTSIi066-A (HPSI0613i-febc_1) WTSIi067-A (HPSI0713i-foop_1) WTSIi067-B (HPSI0713i-foop_2) WTSIi071-A (HPSI0913i-lise_1) WTSIi066-B (HPSI0613i-febc_2) WTSIi012-B (HPSI0314i-bubh_3) WTSIi074-A (HPSI0114i-joxm_1) WTSIi017-B (HPSI0114i-lexy_1) WTSIi075-A (HPSI0114i-vass_1) WTSIi076-A (HPSI0214i-kehc_2) WTSIi077-A (HPSI0314i-cuhk_1) WTSIi011-B (HPSI0314i-qonc_1) |
Provider |
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Depositor | CHDI Foundation Inc. (CHDI) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
External Databases |
|
hPSCreg | CHDIi051-A |
BioSamples | SAMEA4675911 |
ECACC | 66541197 |
Cellosaurus | CVCL_VD22 |
CLO | CLO_0100604 |
Wikidata | Q54811819 |
General Information |
|
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
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Sex | female |
Age of donor (at collection) | 30-34 |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
BioSamples | SAMEA4675910 |
hIPSC Derivation#
General |
|
Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
Age of donor (at collection) | 30-34 |
Passage number reprogrammed | P7 |
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
RT-PCR
|
Vector free reprogramming |
|
Type of used vector free reprogramming factor(s) |
None
|
Other |
|
Selection criteria for clones | Clone morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
SSEA-4 |
Yes |
|||||
TRA 1-60 |
Yes |
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SSEA-1 |
No |
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POU5F1 (OCT-4) |
Yes |
Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |