CHDIi004-A
#4c2, CHDI-90002152
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg Name | CHDIi004-A |
Alternative name(s) |
#4c2, CHDI-90002152
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
WTSIi053-A (HPSI0413i-corn_1) WTSIi054-A (HPSI0413i-nudd_1) WTSIi064-A (HPSI0613i-bohf_1) WTSIi065-A (HPSI0613i-eojr_2) WTSIi068-A (HPSI0613i-giuo_5) WTSIi070-A (HPSI0713i-terl_1) WTSIi072-A (HPSI0114i-fikt_3) WTSIi065-B (HPSI0613i-eojr_3) WTSIi070-B (HPSI0713i-terl_2) WTSIi042-B (HPSI1013i-jufd_3) WTSIi078-A (HPSI0314i-fafq_1) WTSIi079-A (HPSI1013i-hiaf_2) WTSIi081-A (HPSI1113i-qorq_2) WTSIi084-A (HPSI0713i-cicb_3) WTSIi090-A (HPSI0813i-robp_3) WTSIi091-A (HPSI0813i-ffdc_11) WTSIi092-A (HPSI0713i-nemb_1) WTSIi093-A (HPSI0413i-sukz_1) WTSIi095-A (HPSI0813i-vajx_2) WTSIi101-A (HPSI0513i-fejf_2) WTSIi106-A (HPSI0613i-hikj_2) WTSIi107-A (HPSI0813i-iill_1) WTSIi081-B (HPSI1113i-qorq_1) WTSIi084-B (HPSI0713i-cicb_2) WTSIi002-A (HPSI0913i-eika_2) WTSIi004-A (HPSI1113i-qolg_3) WTSIi006-A (HPSI1113i-hayt_1) WTSIi008-A (HPSI1013i-kuxp_1) WTSIi010-A (HPSI0314i-xugn_1) WTSIi013-A (HPSI0214i-kucg_2) WTSIi014-A (HPSI0214i-heja_2) WTSIi016-A (HPSI0114i-oevr_3) WTSIi018-A (HPSI0114i-kolf_3) WTSIi019-A (HPSI0114i-iisa_3) WTSIi022-A (HPSI1013i-yemz_1) WTSIi023-A (HPSI0114i-zapk_3) WTSIi025-A (HPSI1013i-pamv_1) WTSIi031-A (HPSI0314i-bipt_1) WTSIi032-A (HPSI1113i-bima_1) WTSIi039-A (HPSI1013i-wopl_1) WTSIi042-A (HPSI1013i-jufd_2) WTSIi014-B (HPSI0214i-heja_1) WTSIi010-B (HPSI0314i-xugn_2) WTSIi025-B (HPSI1013i-pamv_3) WTSIi004-B (HPSI1113i-qolg_1) WTSIi022-B (HPSI1013i-yemz_3) WTSIi006-B (HPSI1113i-hayt_3) WTSIi019-B (HPSI0114i-iisa_1) WTSIi050-A (HPSI1014i-toss_3) WTSIi051-A (HPSI1113i-uofv_1) |
Provider |
|
Depositor | CHDI Foundation Inc. (CHDI) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
External Databases |
|
hPSCreg | CHDIi004-A |
BioSamples | SAMEA1088035 |
ECACC | 66541150 |
Cellosaurus | CVCL_VC75 |
CLO | CLO_0100592 |
Wikidata | Q54811765 |
General Information |
|
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
|
Sex | male |
Age of donor (at collection) | 30-34 |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
BioSamples | SAMEA1088036 |
hIPSC Derivation#
General |
|
Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
Age of donor (at collection) | 30-34 |
Passage number reprogrammed | P6 |
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
RT-PCR
|
Vector free reprogramming |
|
Type of used vector free reprogramming factor(s) |
None
|
Other |
|
Selection criteria for clones | Clone morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
SSEA-4 |
Yes |
|||||
TRA 1-60 |
Yes |
|||||
SSEA-1 |
No |
|||||
POU5F1 (OCT-4) |
Yes |
Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |