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CENSOi008-A

FB77R2c3, CENSOi260

iPSC line

In Stock
At European Collection of Authenticated Cell Cultures (ECACC)
Timepoint: Confluence
Magnification: x4
Timepoint: Confluence
Magnification: x10
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line
hPSCreg Name CENSOi008-A
Alternative name(s)
FB77R2c3, CENSOi260
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider
Depositor Censo an Axol Bioscience Company (CENSO)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases
hPSCreg CENSOi008-A
BioSamples SAMEA104133816
Cellosaurus CVCL_LE91
ECACC 66540603
CLO CLO_0101674
Wikidata Q54809301

General Information
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information
Sex female
Age of donor (at collection) 25-29

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
myotonic dystrophy type 1
myotonic dystrophy type 1 in the OLS
The donor is a carrier of a disease-associated mutation and affected.
Genetic variants
DMPK (target)
DMPK expansion mutation

Karyotyping (Donor)
Has the donor karyotype been analysed?
No

External Databases (Donor)
BioSamples SAMEA104133817

hIPSC Derivation#

General
Source cell type
fibroblast
fibroblast in the OLS
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 25-29
Collected in 2015

Reprogramming method
Vector type None

Vector free reprogramming
Type of used vector free reprogramming factor(s)
mRNA
mRNA

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch
Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
SSEA-1
No
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
MIXL1
Yes
GATA6
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
NCAM1
Yes
VIM
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
NeuroD1
Yes
HES5
Yes

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility
Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
No autosomal or sex aneuploidies were detected in this sample
Passage number: 10
Karyotyping method: KaryoLite BoBs

Other Genotyping (Cell Line)