BIHi011-A
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
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hPSCreg Name | BIHi011-A |
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
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Provider |
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Depositor | Berlin Institute of Health (BIH) |
Owner | Berlin Institute of Health (BIH) |
Distributors |
Berlin Institute of Health (BIH)
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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Derivation country | Germany |
External Databases |
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hPSCreg | BIHi011-A |
BioSamples | SAMEA104130514 |
ECACC | 66540587 |
Cellosaurus | CVCL_VA03 |
CLO | CLO_0101962 |
Wikidata | Q54796640 |
General Information |
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Publications | View all related publications on hPSCreg (1) |
* Is the cell line readily obtainable for third parties? |
Yes Clinical use: not allowed
Commercial use: allowed
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Donor Information#
General Donor Information |
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Sex | female |
Age of donor (at collection) | 40-44 |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA104130515 |
hIPSC Derivation#
General |
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Source cell type |
peripheral blood mononuclear cellA leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
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Age of donor (at collection) | 40-44 |
Collected in | 2016 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Vector free reprogramming |
|
Other |
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Selection criteria for clones | typical hPSC morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 5 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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Pluripotency Score | Novelty Score | |
32.68 | 1.30 |
Differentiation Potency
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46,XX
Karyotyping method:
KaryoLite BoBs
|
Other Genotyping (Cell Line) |
|
Is there genome-wide genotyping or functional data available? |
Yes
SNP typing array
no mutations detected by virtual karyotyping |