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DHMi005-A-4
L_FLAG Clone 7
Gene-edited iPSC line
The cell line is not released.
General#
Cell Line |
|
| hPSCreg name | DHMi005-A-4 |
| Alternative name(s) |
L_FLAG Clone 7
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
Provider |
|
| Depositor | German Heart Center Munich (DHM) |
| Owner | German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure |
| Distributors |
German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure
|
| Derivation country | Germany |
External Databases |
|
| hPSCreg | DHMi005-A-4 |
| BioSamples | SAMEA14493160 |
| Cellosaurus | CVCL_C0IT |
| Wikidata | Q112929456 |
General Information |
|
| Publications | View all related publications on hPSCreg (1) |
| This EBiSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
| Subclone of | (not in EBiSC, see DHMi005-A in hPSCreg) |
Donor Information#
General Donor Information |
|
| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | No abnormalities have been described with regard to the family history. |
| Is the medical history available upon request? | no, healthy proband |
| Is clinical information available? | no, healthy proband |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA12738677 |
hIPSC Derivation#
General |
|
|
More source cell information can be found in the parental cell line
DHMi005-A in hPSCreg.
|
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
ReLeSR
|
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
In vitro spontaneous differentiation
In vitro spontaneous differentiation
In vitro spontaneous differentiation
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification#
| Genetic modifications not related to a disease |
|