BCRTi009-A
iPSC line
The cell line is not available.
General#
Cell Line |
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| hPSCreg Name | BCRTi009-A |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
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| Depositor | Berlin-Brandenburg Center for Regenerative Therapies (BCRT) |
| Owner | Berlin-Brandenburg Center for Regenerative Therapies (BCRT) |
| Distributors |
Berlin-Brandenburg Center for Regenerative Therapies (BCRT)
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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| Derivation country | Germany |
External Databases |
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| hPSCreg | BCRTi009-A |
| BioSamples | SAMEA112638680 |
| CLO | CLO_0104205 |
| Cellosaurus | CVCL_C7RJ |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
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Donor Information#
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA112638681 |
hIPSC Derivation#
General |
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| Source cell type |
Peripheral Blood Mononuclear CellA peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
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| Source cell origin |
BloodA liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
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| Passage number reprogrammed | 10 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
Yes |
| Methods used |
RT-PCR
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Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
Enzyme-free cell dissociation EDTA
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| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| NANOG |
Yes |
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Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| SOX17 |
Yes |
| Goosecoid |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| Brachyuri (T) |
Yes |
| MIXL1 |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| NEUROD1 |
Yes |
| OTX2 |
Unknown |
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
Molecular Karyotyping by SNP array
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
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