BCRTi006-A
iPSC line
The cell line is not available.
General#
Cell Line |
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hPSCreg Name | BCRTi006-A |
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
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Depositor | Berlin-Brandenburg Center for Regenerative Therapies (BCRT) |
Owner | Berlin-Brandenburg Center for Regenerative Therapies (BCRT) |
Distributors |
Berlin-Brandenburg Center for Regenerative Therapies (BCRT)
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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Derivation country | Germany |
External Databases |
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hPSCreg | BCRTi006-A |
BioSamples | SAMEA112294705 |
CLO | CLO_0104203 |
Cellosaurus | CVCL_C6SR |
Wikidata | Q117704208 |
General Information |
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Publications | View all related publications on hPSCreg (1) |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
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Donor Information#
General Donor Information |
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Sex | female |
Age of donor (at collection) | 45-49 |
Ethnicity | European |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Disease associated phenotypes |
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
No
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External Databases (Donor) |
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BioSamples | SAMEA112294706 |
hIPSC Derivation#
General |
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Source cell type |
kidney epithelial cellAn epithelial cell of the kidney.
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Source cell origin |
UrineThe fluid that is excreted by the kidneys. It is stored in the bladder and discharged through the urethra.
Synonyms
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Age of donor (at collection) | 45-49 |
Passage number reprogrammed | 7 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Episomal |
Genes | |
Is reprogramming vector detectable? |
Yes |
Methods used |
RT-PCR
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Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Type of used vector free reprogramming factor(s) |
None
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Other |
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Selection criteria for clones | Isolated clones were selected based on Epi5 vector loss, staining positive for pluripotency markers and ablility to differentiate into mesoderm, ectoderm and endoderm. |
Derived under xeno-free conditions |
Yes |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzymatically
Enzyme-free cell dissociation EDTA
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O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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NANOG |
Yes |
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TRA 1-60 |
Yes |
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SSEA-4 |
Yes |
Differentiation Potency
In vitro spontaneous differentiation
Marker | Expressed |
SOX17 |
Yes |
Goosecoid |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
MIXL1 |
Yes |
Brachyuri (T) |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
NEUROD1 |
Yes |
OTX2 |
Yes |
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
Molecular Karyotyping by SNP array
Passage number: 12
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |