BCRTi006-A
iPSC line
The cell line is not available.
General#
Cell Line |
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| hPSCreg Name | BCRTi006-A |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
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| Depositor | Berlin-Brandenburg Center for Regenerative Therapies (BCRT) |
| Owner | Berlin-Brandenburg Center for Regenerative Therapies (BCRT) |
| Distributors |
Berlin-Brandenburg Center for Regenerative Therapies (BCRT)
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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| Derivation country | Germany |
External Databases |
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| hPSCreg | BCRTi006-A |
| BioSamples | SAMEA112294705 |
| CLO | CLO_0104203 |
| Cellosaurus | CVCL_C6SR |
General Information |
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| Publications | View all related publications on hPSCreg (1) |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
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Donor Information#
General Donor Information |
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| Sex | female |
| Age of donor (at collection) | 45-49 |
| Ethnicity | European |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Disease associated phenotypes |
|
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA112294706 |
hIPSC Derivation#
General |
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| Source cell type |
kidney epithelial cellAn epithelial cell of the kidney.
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| Source cell origin |
UrineThe fluid that is excreted by the kidneys. It is stored in the bladder and discharged through the urethra.
Synonyms
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| Age of donor (at collection) | 45-49 |
| Passage number reprogrammed | 7 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
Yes |
| Methods used |
RT-PCR
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| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Selection criteria for clones | Isolated clones were selected based on Epi5 vector loss, staining positive for pluripotency markers and ablility to differentiate into mesoderm, ectoderm and endoderm. |
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
Enzyme-free cell dissociation EDTA
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| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| TRA 1-60 |
Yes |
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| SSEA-4 |
Yes |
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| SOX17 |
Yes |
| Goosecoid |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| MIXL1 |
Yes |
| Brachyuri (T) |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| NEUROD1 |
Yes |
| OTX2 |
Yes |
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
Molecular Karyotyping by SNP array
Passage number: 12
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
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