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WTSIi184-A

HPSI1014i-qayj_3

iPSC line

Not-for-profit fee: £1400 per vial
Stock not immediately available - enquire for details
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

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General#

Cell Line

hPSCreg name WTSIi184-A
Alternative name(s)
HPSI1014i-qayj_3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi184-B
(HPSI1014i-qayj_4)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi184-A
BioSamples SAMEA3963885
HipSci HPSI1014i-qayj_3
Cellosaurus CVCL_EE63
Wikidata Q54891596

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 60-64
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2821481
HipSci HPSI-qayj

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 60-64
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
8.622 1.223

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI1014i-qayj_3
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376060
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861267
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447933
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629717
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376201
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860787
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860883
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860403
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560651
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861075
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861171
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368878
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560667
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859923
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860691
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860019
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860499
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860595
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369170
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629727
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560553
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860979
Raw sequencing reads
WGS-derived disease associations
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (SETBP1)
Fanconi anemia complementation group A (FANCA)
focal segmental glomerulosclerosis 9 (CRB2)
Hermansky-Pudlak syndrome 4 (HPS4)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
primary ciliary dyskinesia 7 (DNAH11)
PTEN hamartoma tumor syndrome (PTEN)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
Other WGS-derived genes
AGL, AKR1E2, ALDH3A2, AMPD1, ATXN3, AURKC, BMP4, CARD8, CASP12, CEP170, CHIT1, CHST15, COLQ, CST3, CYP21A2, CYP2D6, CYP2F1, D2HGDH, DEFB126, DNAAF1, DNAH9, DSC3, EME2, FBXO7, FLG2, FUT2, FZD6, GALNT3, GDPD4, IDO2, ITGB2, KCNJ16, LAMA5, LAMB4, MAPT, MICA, MROH8, NDUFB9, NFU1, NOTCH2, NPRL3, OAS1, OR51F1, OR52B4, OTOR, P2RX5, PDE4DIP, PIGN, POLDIP2, POLR3B, PRKRA, PTCHD3, RNF212, RP1L1, RXFP2, SIGLEC12, SLC37A4, SPATA7, SRA1, STAG2, TAP2, TGIF1, TIGD6, TMEM107, TMEM175, TMX3, TNRC18, TOR1AIP1, TREH, TRPM1, WDR37, ZAN, ZNF124, ZNF527