BIONi027-A
H040815 48-1, SAMEA4455499
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
| hPSCreg Name | BIONi027-A |
| Alternative name(s) |
H040815 48-1, SAMEA4455499
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
BIONi013-A (H240715 47-1, SAMEA4342502) BIONi014-A (H270715 47-1, SAMEA4342566) BIONi015-A (H280715 47-1, SAMEA4342649) BIONi016-A (H310715 47-1) BIONi017-A (H030815 47-1, SAMEA4451663) BIONi018-A (H050815 48-4, SAMEA4451665) BIONi019-A (H060815 48-3, SAMEA4451667) BIONi020-A (H070815 47-2, SAMEA4451669) BIONi021-A (H100815 48-2, SAMEA4451671) BIONi022-A (H110815 47-2, SAMEA4451673) BIONi023-A (H130815 47-4, SAMEA4454014) BIONi024-A (H170815 47-2, SAMEA4454016) BIONi025-A (H210815 48-3, SAMEA4454018) BIONi026-A (H300715 48-3) BIONi028-A (H120815 48-1, SAMEA4455501) BIONi029-A (H190815 47-1, SAMEA4455503) BIONi030-A (H200815 47-1, SAMEA4455505) BIONi031-A (H240815 48-2, SAMEA4455507) BIONi032-A (H250815 48-1, SAMEA4455509) BIONi033-A (H180615, SAMEA4563389) BIONi034-A (H290715, SAMEA4563391) BIONi035-A (H180815, SAMEA4563393) WTSIi010-A (HPSI0314i-xugn_1) WTSIi013-A (HPSI0214i-kucg_2) WTSIi014-A (HPSI0214i-heja_2) WTSIi016-A (HPSI0114i-oevr_3) WTSIi018-A (HPSI0114i-kolf_3) WTSIi019-A (HPSI0114i-iisa_3) BIONi010-A (K1P53) BIONi010-B (K2P53, BIONi010-B) BIONi010-C (BIONi010-C, K3P53) RBi001-A (fb101, RB101) WTSIi022-A (HPSI1013i-yemz_1) WTSIi023-A (HPSI0114i-zapk_3) WTSIi025-A (HPSI1013i-pamv_1) WTSIi031-A (HPSI0314i-bipt_1) WTSIi032-A (HPSI1113i-bima_1) HUBi001-A (HUB001Ai) WTSIi039-A (HPSI1013i-wopl_1) WTSIi042-A (HPSI1013i-jufd_2) WTSIi014-B (HPSI0214i-heja_1) UKKi011-A (NP0040-8) UKKi012-A (NP0041-17, NP0041) UKBi002-A (iLB-C2-36m-r2) UKBi004-A (LB-30-12, iLB-C1-30m-r12) UKBi006-A (iLB-C-35m-r1, LB-35-1) WTSIi002-A (HPSI0913i-eika_2) WTSIi004-A (HPSI1113i-qolg_3) WTSIi006-A (HPSI1113i-hayt_1) WTSIi008-A (HPSI1013i-kuxp_1) |
Provider |
|
| Depositor | Bioneer (BION) |
| Owner | Bioneer (BSC) |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
| Derivation country | Denmark |
External Databases |
|
| hPSCreg | BIONi027-A |
| BioSamples | SAMEA4455499 |
| Cellosaurus | CVCL_LE25 |
| ECACC | 66540400 |
| CLO | CLO_0100573 |
| Wikidata | Q54796857 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information#
General Donor Information |
|
| Sex | male |
| Age of donor (at collection) | 20-24 |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Non-disease associated phenotypes |
|
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA4455498 |
hIPSC Derivation#
General |
|
| Source cell type |
adipose tissue derived mesenchymal stem cellAn adult mesenchymal stem cell derived from adipose tisssue.
Synonyms
|
| Source cell origin |
adipose tissuePortion of connective tissue composed of adipocytes enmeshed in areolar tissue.
Synonyms
|
| Source cell type (free text) | Pre-Adipocyte |
| Age of donor (at collection) | 20-24 |
| Passage number reprogrammed | 3 |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
| Vector map | |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
| Culture medium | Essential E8 |
| Passage method | EDTA |
| Surface coating | Matrigel / Geltrex |
| O2 concentration | 5 |
| CO2 concentration | 5 |
| Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
|
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| SOX2 |
Yes |
|
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| SSEA-1 |
No |
|
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| SSEA-4 |
Yes |
|
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| TRA 1-81 |
Yes |
|
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| TDGF1 |
Yes |
|
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| GDF3 |
Yes |
|
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| GABRB3 |
Yes |
|
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| DMNT3B |
Yes |
|
Morphology pictures
Differentiation Potency
In vitro directed differentiation
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Sterility |
|
| Inoculation for microbiological growth | No Contaminants Detected |
| Mycoplasma | Not Detected |
| Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|